Impact of waste dumps of mines on plants in genomic and bio molecules level

Abstract EN

The prime aim of this study was to analyze the effects of mine waste dumps on plants namely J.

curcasandG.

hirsutum, applied to phytoremediation process on waste dumps of magnesite and bauxite mines.

The waste dumps of the mines contain impermissible limits of heavy metals Cd (2070 & 1060) and Pb (443 & 742.6 mg/kg-1), it’s followed by Zn (1141 & 827.5) and Cr (69.96 & 553.7 in magnesite and bauxite mine respectively).

The phytochemical and macro biomolecule analysis showed deviation among the plants in chlorophyll, protein and carbohydrate content.

These plants possess fine phytoremediation potentiality on both mine soil.

A random amplified polymorphic DNA (RAPD) technique was exhibited to detect genotoxin-induced DNA damage of plants from heavy metal contaminated soil (mines) during phytoremediation.

The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands in RAPD profiles with universal 20 operon 10-base pair primers (OPA serious), with 60% – 70% GC content and they showed significant changes happened in plants applied for phytoremediation process on mine waste.

The dendrogram reports are clearly reported the DNA variation in the plants (mutation).

The results showed that RAPD analysis could be a useful tool for detection of genotoxic effects of heavy metal (Cd) toxicity on plants from mine soil.