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Rapid direct detection and differentiation of mycobacterium tuberculosis complex in sputum by real-time PCR
Other Title(s)
الكشف و التمييز المباشر السريع لعصيات السل المركبة في القشع باستخدام تفاعل البوليميراز المتسلسل اللحظي
Joint Authors
Abd al-Sahib, Sabah Sad
al-Fahham, Muhammad Abd al-Ikhwah
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab
Source
Issue
Vol. 56, Issue 4A (31 Dec. 2015), pp.2862-2866, 5 p.
Publisher
University of Baghdad College of Science
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
Tuberculosis status as the second leading causes of significant morbidity and mortality from an infectious disease worldwide, after human immunodeficiency virus (HIV).
Sample collection was conducted at the Institute of Chest and Respiratory Diseases / Baghdad Medical City in Baghdad.
The collection interval was from August to October 2014, 629 suspected TB patients were examined during this period.
The results revealed among total 629 specimens, 56 (8.9%) of the specimens were positive by direct examination and 573 (91.1%) negative specimens by smear microscopy.
Fifty six DNA samples were extracted from positive ZN smears of sputum specimens and 40 samples from healthy persons (as control) were subjected to molecular diagnosis by real time PCR to detect and differentiate of M.
tuberculosis, M.
bovis and M.
bovis BCG.
The results were clarified that the 48 samples (85.72%) were M.
tuberculosis, 2 samples (3.57%) were mixed of M.
tuberculosis and M.
bovis, no M.
bovis BCG was detected, and 6 (10.71%) were negative.
These findings propose that M.
bovis plays a minor role compared to M.
tuberculosis in the etiology of pulmonary tuberculosis in Baghdad.
American Psychological Association (APA)
Abd al-Sahib, Sabah Sad& al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab& al-Fahham, Muhammad Abd al-Ikhwah. 2015. Rapid direct detection and differentiation of mycobacterium tuberculosis complex in sputum by real-time PCR. Iraqi Journal of Science،Vol. 56, no. 4A, pp.2862-2866.
https://search.emarefa.net/detail/BIM-651768
Modern Language Association (MLA)
Abd al-Sahib, Sabah Sad…[et al.]. Rapid direct detection and differentiation of mycobacterium tuberculosis complex in sputum by real-time PCR. Iraqi Journal of Science Vol. 56, no. 4A (2015), pp.2862-2866.
https://search.emarefa.net/detail/BIM-651768
American Medical Association (AMA)
Abd al-Sahib, Sabah Sad& al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab& al-Fahham, Muhammad Abd al-Ikhwah. Rapid direct detection and differentiation of mycobacterium tuberculosis complex in sputum by real-time PCR. Iraqi Journal of Science. 2015. Vol. 56, no. 4A, pp.2862-2866.
https://search.emarefa.net/detail/BIM-651768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2865-2866
Record ID
BIM-651768