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Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean
Joint Authors
Zarkr, Sajad al-Majid
Farahat, Sufia
Mahajan, Ritika
Bakri, Ayushi
Sharma, Arjun
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 1 (31 Jan. 2016), pp.139-149, 11 p.
Publisher
Publication Date
2016-01-31
Country of Publication
Saudi Arabia
No. of Pages
11
Main Subjects
Topics
Abstract EN
Increase in food production viz-a-viz quality of food is important to feed the growing human population to attain food as well as nutritional security.
The availability of diverse germplasm of any crop is an important genetic resource to mine the genes that may assist in attaining food as well as nutritional security.
Here we used 15 RAPD and 23 SSR markers to elucidate diversity among 51 common bean genotypes mostly landraces collected from the Himalayan region of Jammu and Kashmir, India.
We observed that both the markers are highly polymorphic.
The discriminatory power of these markers was determined using various parameters like; percent polymorphism, PIC, resolving power and marker index.
15 RAPDs produced 171 polymorphic bands, while 23 SSRs produced 268 polymorphic bands.
SSRs showed a higher PIC value (0.300) compared to RAPDs (0.243).
Further the resolving power of SSRs was 5.241 compared to 3.86 for RAPDs.
However, RAPDs showed a higher marker index (2.69) compared to SSRs (1.279) that may be attributed to their higher multiplex ratio.
The dendrograms generated with hierarchical UPGMA cluster analysis grouped genotypes into two main clusters with various degrees of sub clustering within the cluster.
Here we observed that both the marker systems showed
American Psychological Association (APA)
Zarkr, Sajad al-Majid& Farahat, Sufia& Mahajan, Ritika& Bakri, Ayushi& Sharma, Arjun. 2016. Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean. Saudi Journal of Biological Sciences،Vol. 23, no. 1, pp.139-149.
https://search.emarefa.net/detail/BIM-652110
Modern Language Association (MLA)
Zarkr, Sajad al-Majid…[et al.]. Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean. Saudi Journal of Biological Sciences Vol. 23, no. 1 (Jan. 2016), pp.139-149.
https://search.emarefa.net/detail/BIM-652110
American Medical Association (AMA)
Zarkr, Sajad al-Majid& Farahat, Sufia& Mahajan, Ritika& Bakri, Ayushi& Sharma, Arjun. Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 1, pp.139-149.
https://search.emarefa.net/detail/BIM-652110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 148-149
Record ID
BIM-652110