Photo activated disinfection (PAD)‎ of dental root canal system : an ex-vivo study

Abstract EN

Aim: To investigate the efficacy of photo activated disinfection (PAD) in reducing colony-forming unit (CFU) counts of Enterococcus faecalis (E.

faecalis) in infected dental root canals.

The study compared the efficacy of PAD with conventional endodontic treatment (CET) and also a combination of CET along with PAD.

Material and Methods : 53 maxillary incisors were taken for the study.

Teeth were divided into 3 groups, CET (Group I) (n = 11), PAD (Group II) (n = 21), and a combination of CET and PAD (Group III) which consisted of (n = 21) samples, Group II and Group III were further divided into 2 subgroups, Group IIa, IIb and Group IIIa, IIIb.

Strains of E.

faecalis were inoculated in all the root canals.

CET group samples were treated by chemo-mechanical preparation (CMP) alone, PAD samples were treated with laser alone at 2 different exposure time (4 min and 2 min).

In the combination treatment, samples were treated initially by CET and then by PAD for a time period of 4 min and 2 min.

Contents of the root canal were aspirated, diluted and plated in Tryptone Soya Broth (TSB) and plates were incubated for 24 h to observe the bacterial regrowth.