Urinary catheter indwelling clinical pathogen biofilm formation, exopolysaccharide characterization and their growth influencing parameters
Joint Authors
Murugan, Kasi
Selvanayaki, Krishnasamy
al-Suhaybani, Salih
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 1 (31 Jan. 2016), pp.150-159, 10 p.
Publisher
Publication Date
2016-01-31
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Topics
Abstract EN
Self-reproducing microbial biofilm community mainly involved in the contamination of indwelling medical devices including catheters play a vital role in nosocomial infections.
The catheter-associated urinary tract infection (CA-UTI) causative Staphylococcus aureus, Enterobacter faecalis, and Pseudomonas aeruginosa were selectively isolated, their phenotypic as well as genotypic biofilm formation, production and monomeric sugar composition of EPS as well as sugar, salt, pH and temperature influence on their in vitro biofilm formation were determined.
From 50 culture positive urinary catheters S.
aureus (24%), P.
aeruginosa (18%), E.
faecalis (14%) and others (44%) were isolated.
The performed assays revealed their varying biofilm forming ability.
The isolated S.
aureus ica, E.
faecalis esp, and P.
aeruginosa cup A gene sequencing and phylogenetic analysis showed their close branching and genetic relationship.
The analyzed sugar, salt, pH, and temperature showed that the degree of CA-UTI isolates biofilm formation is an environmentally sensitive process.
EPS monosaccharide HPLC analysis showed the presence of neutral sugars (ng/ll) as follows: glucose (P.
aeruginosa: 44.275; E.
faecalis: 4.23), lactose (P.
aeruginosa: 7.29), mannitol (P.
aeruginosa: 2.53; S.
aureus: 2.62; E.
faecalis: 2.054) and maltose (E.
faecalis: 7.0042
American Psychological Association (APA)
Murugan, Kasi& Selvanayaki, Krishnasamy& al-Suhaybani, Salih. 2016. Urinary catheter indwelling clinical pathogen biofilm formation, exopolysaccharide characterization and their growth influencing parameters. Saudi Journal of Biological Sciences،Vol. 23, no. 1, pp.150-159.
https://search.emarefa.net/detail/BIM-652315
Modern Language Association (MLA)
Murugan, Kasi…[et al.]. Urinary catheter indwelling clinical pathogen biofilm formation, exopolysaccharide characterization and their growth influencing parameters. Saudi Journal of Biological Sciences Vol. 23, no. 1 (Jan. 2016), pp.150-159.
https://search.emarefa.net/detail/BIM-652315
American Medical Association (AMA)
Murugan, Kasi& Selvanayaki, Krishnasamy& al-Suhaybani, Salih. Urinary catheter indwelling clinical pathogen biofilm formation, exopolysaccharide characterization and their growth influencing parameters. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 1, pp.150-159.
https://search.emarefa.net/detail/BIM-652315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 158-159
Record ID
BIM-652315