Genetic diversity using random amplified polymorphic DNA (RAPD)‎ analysis for Aspergillus niger isolates

Abstract EN

This study includes isolation, purification, and identification of Aspergillus niger from different sources (soil, Seeds, powdered milk and factory waste water) by traditional methods (macroscopic and microscopically).

and study the relationships of genetic among the A.

niger isolates based PCR (RAPD) technique (find the DNA fingerprint).

The results of this study revealed that genetic diversity and relationships among twenty four isolates were determined by using the random amplified polymorphic DNA (RAPD) technique.

Genomic DNA of each isolates was extract at final concentration of (115-959) μg /l per (2-3) gm of wet mycelium, and at a purity (1.7-1.9).

Each DNA of isolates were amplified with each 24 primers and the products were resolved electrophoretically on 1.2% agarose gel, stained with ethidium bromide and photographed under UV light.

Ten primers failed to amplify DNA of A.

niger isolates but the rest 14 primers produced 367 bands, of these bands 99.4% (365) bands were polymorphic.

The size of the amplified bands ranged between 130-2840 bp.

The genetic polymorphism value of each primer was determined and ranged between 86-100%.

All primer have given unique bands.

The primers were fingerprinted 21 of the 24 isolates.

Genetic distances ranged from 0.11496 to 0.68088 between A.

niger isolates.

Also, cluster analysis of genetic distances between these isolates have divided into groups according to the source, which isolated them and this indicates the presence of a high level of genetic kinship samples isolated from the same source at the same time which indicates the presence of a high level of genetic distance between samples isolated from different sources