Molecular characterization of mycoplasma contaminating tissue cultures

Other Title(s)

التصنيف الجزيئي للميكوبلازما الملوثة لخلايا الزرع النسيجي

Joint Authors

Sad, Ashraf A.
al-Shafii, Dina Y. H.
Abu Shamah, Usamah Hasan

Source

Assiut Veterinary Medical Journal

Issue

Vol. 61, Issue 147 (31 Oct. 2015), pp.135-143, 9 p.

Publisher

Assiut University Faculty of Veterinary Medicine

Publication Date

2015-10-31

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Medicine

Abstract EN

One of the main problems in cell culture unit is mycoplasma contamination.

It can extensively affect cell physiology and metabolism.

As the applications of cell culture increase in research, industrial production, cell therapy and its importance for diagnostic routine work in selected labs, so more concerns about mycoplasma contamination will arise.

In our study, a total of 50 cell cultures from Animal Health Research Institute, Dokki, Giza., VACSERA and Veterinary Serum & Vaccine institute Abbasia were monitored for mycoplasma using culture and PCR methodology.

The contamination was detected in the cell culture collected from all laboratories.

Mycoplasmas were detected by culture in 29/50 (58%) of the cell culture samples which subsequently identified with PCR using Mycoplasma group specific primer detecting all species of mycoplasma which gave bands at 280 bp in all positive culture samples, the most frequent species was M.

arginini (30%), followed by M.

orale (28%).

MDBK were positive only for M.

arginini which gave band at 326 bp, while VERO and BHK cells were infected only with M.

orale which gave bands at 87bp.

Awareness about the sources of mycoplasma and pursuing aseptic techniques in cell culture unit along with reliable detection methods of mycoplasma contamination can provide an appropriate situation to prevent mycoplasma contamination in cell culture.

American Psychological Association (APA)

al-Shafii, Dina Y. H.& Abu Shamah, Usamah Hasan& Sad, Ashraf A.. 2015. Molecular characterization of mycoplasma contaminating tissue cultures. Assiut Veterinary Medical Journal،Vol. 61, no. 147, pp.135-143.
https://search.emarefa.net/detail/BIM-653760

Modern Language Association (MLA)

al-Shafii, Dina Y. H.…[et al.]. Molecular characterization of mycoplasma contaminating tissue cultures. Assiut Veterinary Medical Journal Vol. 61, no. 147 (Oct. 2015), pp.135-143.
https://search.emarefa.net/detail/BIM-653760

American Medical Association (AMA)

al-Shafii, Dina Y. H.& Abu Shamah, Usamah Hasan& Sad, Ashraf A.. Molecular characterization of mycoplasma contaminating tissue cultures. Assiut Veterinary Medical Journal. 2015. Vol. 61, no. 147, pp.135-143.
https://search.emarefa.net/detail/BIM-653760

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 141-143

Record ID

BIM-653760