Pilot study for early prognosis of Azoospermia in relation to Y-STR Profiling
Author
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 17, Issue 1 (31 Jan. 2016), pp.47-56, 10 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2016-01-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract EN
Background: Azoospermia constitutes 20% of male infertility situations and affects 1% of the total male population (Jarvi et al., 2010).
This condition is classified into three major types; pre-testicular, testicular and post-testicular Azoospermia (Sermondade et al., 2012).
Genetic defects causing Azoospermia are due to chromosomal or non-chromosomal alterations on the Y-Chromosome (Lee et al., 2011).
Initial diagnosis of Azoospermia is established when no spermatozoa are detected on microscopic examination of semen (World Health Organisation, 1999).
Objective: To evaluate the correlation of Y-STR Profiling results and the prevalence of Azoospermia, to help for early prognosis of Azoospermia before puberty.
Methods: Buccal swab samples were taken from two groups of individuals (50 fertile and 50 Azoospermic patients), then DNA was isolated using QIAamp DNA Micro kit.
DNA quantification was done using a Real-time PCR utilizing Quantifiler Kit.
PCR was done using PowerPlex Y PCR Amplification Kit, then amplified products were typed using a 3130 Genetic Analyzer.
Results: Five haplotypes in four different Y-STR loci were found to possess significantly higher occurrence percentages in Azoospermic than in fertile Saudi individuals, which can serve as a group of pre-diagnostic markers for early prognosis of Azoospermia in Saudi population.
Conclusion: There was a significant correlation of Y-STR Profiling results and the prevalence of Azoospermia condition, which supports the idea of using Y-STR Profiling in early prognosis of Azoospermia.
American Psychological Association (APA)
Rifat, Ahmad M.. 2016. Pilot study for early prognosis of Azoospermia in relation to Y-STR Profiling. The Egyptian Journal of Medical Human Genetics،Vol. 17, no. 1, pp.47-56.
https://search.emarefa.net/detail/BIM-655056
Modern Language Association (MLA)
Rifat, Ahmad M.. Pilot study for early prognosis of Azoospermia in relation to Y-STR Profiling. The Egyptian Journal of Medical Human Genetics Vol. 17, no. 1 (Jan. 2016), pp.47-56.
https://search.emarefa.net/detail/BIM-655056
American Medical Association (AMA)
Rifat, Ahmad M.. Pilot study for early prognosis of Azoospermia in relation to Y-STR Profiling. The Egyptian Journal of Medical Human Genetics. 2016. Vol. 17, no. 1, pp.47-56.
https://search.emarefa.net/detail/BIM-655056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 55-56
Record ID
BIM-655056