Serological and molecular studies on multi-drug resistant salmonella isolated from captive budgerigars (Melopsittacus undulatus)‎

Abstract EN

The present work was carried out for serological and molecular screening of virulence genes associated with Salmonella in captive budgerigars.

A total of 805 apparently healthy birds were collected from different sources, and subjected to clinical and bacteriological examination.

Salmonella species were isolated at rate of (4.97%) with recognition of 4 different serovars in which Salmonella Paratyphi A was the most common isolated serotype.

All isolates were highly sensitive to Ciprofloxacin, Enrofloxacin and Norfloxacin.

Multiplex-PCR using (invA, spvC and stn) was devised to confirm the isolates and predict their virulence.

(invA;284bp) was detected in all Salmonella isolates with (100%), while (stn; 617bp) and (spvC; 392bp) were detected in some Salmonella isolates.