Diagnostic investigation of salmonella in captive budgerigars using standard microbiological techniques and inva gene specific PCR
Other Title(s)
الفحص التشخيصي للسالمونيلا في طيور الدر الاسترالية الأسيرة باستخدام الطرق التقليدية و تفاعل البلمرة المتسلسل مع الرجوع إلى تغيرات فصول السنة
Joint Authors
Ahmad, Atif Muhammad Kamil
Khafaji, Ahmad Ahmad Rifat
Sad, Inas Muhammad
Jamal al-Din, Muhammad Abd al-Halim Mustafa
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 20, Issue 1 (30 Jun. 2015), pp.147-158, 12 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2015-06-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Topics
Abstract EN
A total of 384 faecal samples were collected from apparently healthy budgerigars and processed to investigate the status of Salmonella in captive budgerigars by Standard Microbiological Techniques (SMT) and PCR with invA gene.
(10.42%) were Salmonella positive by SMT while (18.49%) were positive by PCR.
The incidence by SMT was (11.31%, 12.50% and 6.25%) while, by PCR (25%, 16.67% and 9.38%) in zoos, pet shops and household groups, respectively.
The sensitivity and specificity of the PCR were (100% and 91%) respectively.
Individual samples that examined by SMT and PCR were compared with Pooling method which considered as initial screening method that eliminates all Salmonella-negative samples.
Enriched faecal broth and PCR with invA gene can be used as rapid method for direct detection of Salmonella in the faecal samples of carrier captive birds.
Salmonella isolation rate was (87.5%, 7.5%, 5% and 0%) in Spring, Summer, Winter and Autumn respectively.
American Psychological Association (APA)
Sad, Inas Muhammad& Ahmad, Atif Muhammad Kamil& Khafaji, Ahmad Ahmad Rifat& Jamal al-Din, Muhammad Abd al-Halim Mustafa. 2015. Diagnostic investigation of salmonella in captive budgerigars using standard microbiological techniques and inva gene specific PCR. Suez Canal Veterinary Medicine Journal،Vol. 20, no. 1, pp.147-158.
https://search.emarefa.net/detail/BIM-655089
Modern Language Association (MLA)
Khafaji, Ahmad Ahmad Rifat…[et al.]. Diagnostic investigation of salmonella in captive budgerigars using standard microbiological techniques and inva gene specific PCR. Suez Canal Veterinary Medicine Journal Vol. 20, no. 1 (2015), pp.147-158.
https://search.emarefa.net/detail/BIM-655089
American Medical Association (AMA)
Sad, Inas Muhammad& Ahmad, Atif Muhammad Kamil& Khafaji, Ahmad Ahmad Rifat& Jamal al-Din, Muhammad Abd al-Halim Mustafa. Diagnostic investigation of salmonella in captive budgerigars using standard microbiological techniques and inva gene specific PCR. Suez Canal Veterinary Medicine Journal. 2015. Vol. 20, no. 1, pp.147-158.
https://search.emarefa.net/detail/BIM-655089
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 153-156
Record ID
BIM-655089