Detection of white spot syndrome virus in cultured penaeid shrimp using histopathological observation and polymerase chain reaction

Other Title(s)

الكشف عن مرض البقع البيضاء في الجمبري : فحص الصفة التشريحية المرضية و تفاعل البلمرة المتسلسل

Joint Authors

al-Shahidi, Muhammad Said M.
Thabit, Randa Yusra
Abd al-Wahhab, Shahirah Abd al-Fattah
Abd al-Dayim, Muhammad Mustafa
al-Jamal, Rifat Muhammad Ali
al-Dasuqi, Aminah Ali

Source

Suez Canal Veterinary Medicine Journal

Issue

Vol. 20, Issue 1 (30 Jun. 2015), pp.213-226, 14 p.

Publisher

Suez Canal University Faculty of Veterinary Medicine

Publication Date

2015-06-30

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Biology
Zoology

Topics

Abstract EN

White spot syndrome virus (WSSV) in Egyptian shrimp is poorly understood and there is no available information regarding possible WSSV infection of native shrimp in Egypt.

Therefore, the main goal of this work was to diagnose WSSV in Egyptian shrimp by histopathological examination and molecular based methods (PCR).

A total of 90 shrimp samples showed circular white patches in the cuticle were collected from the local fish markets of high shrimp producing region in three provinces of Egypt.

The three regions located at the south coast of Mediterranean Sea, including El-Arish, Dumiatta and Kafr El-sheikh.

Histopathological examination of different organs showed intranuclear basophilic inclusion bodies.

Single-step PCR results with primers specific for the gene encoding VP28 gave a predicted amplified product at 677 bp.

The primer pair designed from WSSV genomic sequence yields a 677 bp fragment by agarose gel electrophoresis

American Psychological Association (APA)

al-Shahidi, Muhammad Said M.& al-Jamal, Rifat Muhammad Ali& al-Dasuqi, Aminah Ali& Thabit, Randa Yusra& Abd al-Wahhab, Shahirah Abd al-Fattah& Abd al-Dayim, Muhammad Mustafa. 2015. Detection of white spot syndrome virus in cultured penaeid shrimp using histopathological observation and polymerase chain reaction. Suez Canal Veterinary Medicine Journal،Vol. 20, no. 1, pp.213-226.
https://search.emarefa.net/detail/BIM-655131

Modern Language Association (MLA)

al-Jamal, Rifat Muhammad Ali…[et al.]. Detection of white spot syndrome virus in cultured penaeid shrimp using histopathological observation and polymerase chain reaction. Suez Canal Veterinary Medicine Journal Vol. 20, no. 1 (2015), pp.213-226.
https://search.emarefa.net/detail/BIM-655131

American Medical Association (AMA)

al-Shahidi, Muhammad Said M.& al-Jamal, Rifat Muhammad Ali& al-Dasuqi, Aminah Ali& Thabit, Randa Yusra& Abd al-Wahhab, Shahirah Abd al-Fattah& Abd al-Dayim, Muhammad Mustafa. Detection of white spot syndrome virus in cultured penaeid shrimp using histopathological observation and polymerase chain reaction. Suez Canal Veterinary Medicine Journal. 2015. Vol. 20, no. 1, pp.213-226.
https://search.emarefa.net/detail/BIM-655131

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 222-226

Record ID

BIM-655131