![](/images/graphics-bg.png)
Study the ability of Enterotoxigenic E.coli antigens to induce cellular immune response by erythrocyte-rosette formation and leukocyte migration inhibition factor in rabbits
Other Title(s)
دراسة قابلية مستضدات بكتريا الأشريكيا القولونية المنتجة للسموم المعوية على تحفيز الاستجابة المناعية الخلوية بواسطة التشكيل الزهري الثاني و معامل التثبيط هجرة الخلايا البيض في الأرانب
Joint Authors
al-Jamil, Diya S. A.
al-Shar, Jamal M. R.
Abd al-Zahrah, Ala Abd al-Husayn
Source
Kufa Journal for Nursing Sciences
Issue
Vol. 5, Issue 3 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
University of Kufa Faculty of Nursing \
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Objectives :This study was aimed to investigate the ability of Enterotoxigenic E.coli and their enterotoxins to induce cellular immune response by means of erythrocyte-rosette formation and leukocyte migration inhibition factor tests.
Methods: Total of 30 stool sample was collected from patient with severe diarrhea whom attended to Al-Sadder teaching hospital in Al-Najaf city in period extended from April to June 2013.
All bacterial isolates was identified according to morphological and biochemical testes and by API 20 E system, which further conformational tested for heat stable enterotoxin production by Suckling Mice Assay (SMA).
three antigens (heat killed bacterial cell, crude enterotoxin and purified heat stable enterotoxin ETECST) was prepared and injected in experimental animals(rabbit) and tested in the level of systemic and mucosa (appendix and duodenum).
Statistical analysis was done by least significant difference (LSD).
Results: The results showed that 20% (6 isolates) was Enterotoxigenic E.coli and the immune experiments showed significant increase level of E-rosette formation in rabbit injected with heat killed antigen (50.1%, 49.7% and 53.0%) compared with control (23.1%, 31.8% and 29.6%) in blood appendix and duodenum consecutively, while high level appeared in crude enterotoxin and ETECST in appendix (41.3%) and (47.4%) consecutively.
The assessment of ETECST LIF in migration percent to sensitizer was showing high significant LIF (54.5%, 47.6% and 43.3%) compared with control (82.0%, 79.5% and 79.5%) in blood appendix and duodenum consecutively.
Conclusions: crud and partially purified enterotoxins have ability to induce cellular immune response in blood and mucosa.
Recommendation: prepare a carrier molecule model for partially purified enterotoxins to make a vaccine against Enterotoxigenic E.coli .
American Psychological Association (APA)
al-Jamil, Diya S. A.& Abd al-Zahrah, Ala Abd al-Husayn& al-Shar, Jamal M. R.. 2015. Study the ability of Enterotoxigenic E.coli antigens to induce cellular immune response by erythrocyte-rosette formation and leukocyte migration inhibition factor in rabbits. Kufa Journal for Nursing Sciences،Vol. 5, no. 3, pp.1-9.
https://search.emarefa.net/detail/BIM-655557
Modern Language Association (MLA)
al-Jamil, Diya S. A.…[et al.]. Study the ability of Enterotoxigenic E.coli antigens to induce cellular immune response by erythrocyte-rosette formation and leukocyte migration inhibition factor in rabbits. Kufa Journal for Nursing Sciences Vol. 5, no. 3 (Sep. / Dec. 2015), pp.1-9.
https://search.emarefa.net/detail/BIM-655557
American Medical Association (AMA)
al-Jamil, Diya S. A.& Abd al-Zahrah, Ala Abd al-Husayn& al-Shar, Jamal M. R.. Study the ability of Enterotoxigenic E.coli antigens to induce cellular immune response by erythrocyte-rosette formation and leukocyte migration inhibition factor in rabbits. Kufa Journal for Nursing Sciences. 2015. Vol. 5, no. 3, pp.1-9.
https://search.emarefa.net/detail/BIM-655557
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8-9
Record ID
BIM-655557