Molecular detection of hydatid cyst isolated from goats in al-Qadisiyia Province by polymerase chain reaction
Author
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 6, Issue 1 (30 Jun. 2015), pp.191-195, 5 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
The present study was conducted during the period from August 2013 to April 2014 in AL-Qadisiyia province to detect the infection of hydatidiosis in Goat using Molecular and conventional methods.
One hundred and seventy nine Goat were examined at slaughters in Aldewanyia abattoir.
Caprine hydatid cyst were removed from infected liver and lung after visceral inspection of these organs then investigated the viability of protoscolices by staining with eosin stain then examining under light microscope of 40x.The results showed 45 hydatid cyst were found from 179 goat , the prevalence rate of infection (25.13 %) .
20 cyst were found fertile only whereas 25 cyst were sterile.
Regarding to infection site in organs, out of 45 hydatid cyst collected , 28(62.22 %) cyst were isolated from liver whereas 17 (37.77 %) isolated from lung with significant differences at p<0.05.
Out of 45 hydatid cyst samples were taken from infected goats, and examined by conventional PCR , the PCR product demonstrated an expected fragment of 440 bp in the length , 10samples were given positive cases , 7(25 %) samples from liver whereas only 3(12 %) from lung samples gave positive PCR result, with statistically significant difference (P < 0.05) between two organs.
American Psychological Association (APA)
al-Khalidi, Mansur Jadan Ali. 2015. Molecular detection of hydatid cyst isolated from goats in al-Qadisiyia Province by polymerase chain reaction. Kufa Journal for Veterinary Medical Sciences،Vol. 6, no. 1, pp.191-195.
https://search.emarefa.net/detail/BIM-655965
Modern Language Association (MLA)
al-Khalidi, Mansur Jadan Ali. Molecular detection of hydatid cyst isolated from goats in al-Qadisiyia Province by polymerase chain reaction. Kufa Journal for Veterinary Medical Sciences Vol. 6, no. 1 (Jun. 2015), pp.191-195.
https://search.emarefa.net/detail/BIM-655965
American Medical Association (AMA)
al-Khalidi, Mansur Jadan Ali. Molecular detection of hydatid cyst isolated from goats in al-Qadisiyia Province by polymerase chain reaction. Kufa Journal for Veterinary Medical Sciences. 2015. Vol. 6, no. 1, pp.191-195.
https://search.emarefa.net/detail/BIM-655965
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1194-195
Record ID
BIM-655965