Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey
Other Title(s)
استخدام أنزيم البيتا غالاكتوزيداز المستخلص من بعض النباتات في تحضير حليب و مصل حليب خالي من اللاكتوز
Dissertant
Thesis advisor
Comitee Members
al-Rawashidah, Ibrahim Muhammad
al-Quran, Salih Ahmad
al-Tarawinah, Khalid Ahmad
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2015
English Abstract
Crude β-galactosidase extracts of Zingiber officinale and Alliumn sativum were investigated for the activity β-galactosidase.
The enzyme in the extract was characterized in term of pH, temperature and enzyme kinetic.
The crude β-galactosidase extract of Zingiber officinale and Alliumn sativum used also in hydrolysis of lactose in milk and whey.
The enzyme activity was measured by its ability to hydrolyze the substrate 2-nitrophenyl β -D-galactopyranoside (ONPG).
The crude enzyme extract of Zingiber officinale was found to has maximum activity at pH =5.0 and 60°C.
While extract of Alliumn sativum was found to has maximum activity at pH=4.0 and 500C.
Enzyme kinetics in both extracts were indicated to have a potential activities for example, the km and Vmax values of enzyme extract of Zingiber officinale were 2.08 mM and 0.55µmol/min, respectively while 3.2mM and 1.1 µmol/min, respectively in Alliumn sativum.
The application of crude enzyme extract of both plants for lactose hydrolysis in milk showed a significant decreased in the lactose content 30-45% in raw milk, while 50-60% of lactose hydrolyzed in skimmed milk.
The findings indicate the potential use crude enzyme extract of Zingiber officinale and Alliumn sativum for the production of low/delactosed milk for lactose-intolerant population.
However, 55-60% of lactose hydrolyzed in whey.
These results encourage to use their crude enzyme extract in food industry and in manufacturing syrup sweeteners.
Main Subjects
Topics
No. of Pages
44
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
al-Nuaymat, Ali Mudhi. (2015). Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656343
Modern Language Association (MLA)
al-Nuaymat, Ali Mudhi. Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656343
American Medical Association (AMA)
al-Nuaymat, Ali Mudhi. (2015). Using crude β-galactosidase extracts of some plants in preparation of delactosed milk and whey. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656343
Language
English
Data Type
Arab Theses
Record ID
BIM-656343
