Isolation of chitinolytic bacteria and production of chitinase
Dissertant
Thesis advisor
Comitee Members
al-Laymun, Muhammad Awdah
al-Tarawinah, Khalid Ahmad
al-Nuaymat, Sulayman Muhammad
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2015
English Abstract
Chitinolytic enzymes are receiving attention due to their role in biocontrol, medicine and agriculture.
A total of 20 strains of bacterial isolates with extracellular chitinolytic activity were isolated from 50 different dead arthropods collected from different regions of AL-Tafilah, South Jordan.
Bacterial isolate A7 was found to produce the chitinase with higher activity and biocontrol property.
Isolate A7 was identified biochemically and morphologically as well as by 16S rRNA gene sequencing as Bacillus atrophaeus.
Cultivation of B.
atrophaeus in chitin liquid medium resulted in production of chitinase with total activity of 68.9 mU/ml and specific activity of 2670 mU/mg protein.
Calculation of enzyme activity was carried out by DNS method.
The enzyme retained its activity at temperature between 20 ºC to 50 ºC with optimum activity at 40 ºC.
The enzyme was most active between pH 4 and pH 9.
It was relatively stable between at neutral pH ranges, beyond these pH ranges, its activity was rapidly decrease or lost.
This study pointed out the importance of microbial chitinase in controlling insect development and metamorphosis.
Chitinase enzyme produced by B.
atrophaeus A7 inhibits development of D.
melanogaster larvae and it pupation with LD50 (24.1 ± 0.9 mU/ml) and delay the development at low concentrations.
Main Subjects
Topics
No. of Pages
47
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Design and methodology.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
al-Qawabiah, Atiyyah Adnan. (2015). Isolation of chitinolytic bacteria and production of chitinase. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656378
Modern Language Association (MLA)
al-Qawabiah, Atiyyah Adnan. Isolation of chitinolytic bacteria and production of chitinase. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656378
American Medical Association (AMA)
al-Qawabiah, Atiyyah Adnan. (2015). Isolation of chitinolytic bacteria and production of chitinase. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656378
Language
English
Data Type
Arab Theses
Record ID
BIM-656378
