Prevalence of tetracycline resistant Aeromonas Hydrophila isolated from diarrheic patients in Hilla City, Iraq

Abstract EN

The aim of this study was to investigate the prevalence of tetracycline resistant Aeromonashydrophila isolated from clinical sources in Hilla city, Iraq.

A total of 822 samples were collected from fecal specimens from patients.

Samples were collected from those who suffering from diarrhea.

The period of the research was from October 2013 to February 2014 at public health lab, Hilla city.

Results of this study revealed that out of 822 fecal samples, 13 isolates (1.58%) were belonged to Aeromonas spp.

However, other bacterial isolates belonged to other genera similar to Aeromonaswere also recovered.

Out of 13 Aeromonas spp., eight A.

hydrophilaisolates (61.53%) were obtained, while the other isolates were distributed as: four isolates of A.

salmoncidia (30.76%), and one of A.

sobria.

Isolation and detection of A.

salmoncidia species was first recorded in Iraq.

The susceptibly of A.

hydrophila (NO.

8) to several antibiotics was determined using disk diffusion test (DDT).

Results showed that 5 isolates (62.5%) were Multi-drug resistant (MDR) and three isolates (S2, S3, and S5,) were sensitive to most of antibiotic classes tested.

The MIC of A.

hydrophila to tetracycline was also detected using ager dilution mothed according to CLSI guidelines.

Results found that only 2 isolates (25 %) were resistant to tetracycline.

The MIC of these isolates ranged from 0.25-16 μg/ml.

This result confirms tetracycline resistance by these isolates when tested using DDT.