Molecular diagnosis of coccidioides posadasii from aborted placenta of ewes by RT-PCR

Other Title(s)

التشخيص الجزيئي لفطر Coccidioides Posadasii من المشائم المجهضة للنعاج باستخدام تقنية RT-PCR

Joint Authors

Majbil, Hamid Abd al-Husayn
Abd al-Majid, Ban Abbas
Faris, Bushra Hamzah

Source

Kufa Journal for Veterinary Medical Sciences

Issue

Vol. 5, Issue 2 (31 Dec. 2014), pp.24-31, 8 p.

Publisher

University of Kufa Faculty of Veterinary Medicine

Publication Date

2014-12-31

Country of Publication

Iraq

No. of Pages

8

Main Subjects

Veterinary Medicine

Topics

Abstract EN

Diagnosis of diseases depends on histology using formaldehyde-fixed paraffin embedded tissue .The polymerase chain reaction (PCR)helps the pathologists to confirm the diagnosis of many disease including mycotic infections.

Aborted placentas from 40 ewes were formalin fixed, paraffin embedded and sectioned.

Tissue sections were stained with haematoxylin and eosine, and periodic acid Schiff (PAS) for histopathology examination.

Fresh tissue parts were cultivated ,subjected to DNA extraction for the purpose of real-time PCR amplification.

Histopathological examination of placental tissue revealed extensive necrotizing placentitis with the presence of spherule of Coccidioides Posadasiiin the necrotic area.

Only eight samples gave positive growth for coccidioides Posadasii out of forty aborted placental samples.

DNA amplification by real-time PCR revealed positive amplification for the eight samples which confirmed the detection of coccidiodes posadasii.

American Psychological Association (APA)

Faris, Bushra Hamzah& Abd al-Majid, Ban Abbas& Majbil, Hamid Abd al-Husayn. 2014. Molecular diagnosis of coccidioides posadasii from aborted placenta of ewes by RT-PCR. Kufa Journal for Veterinary Medical Sciences،Vol. 5, no. 2, pp.24-31.
https://search.emarefa.net/detail/BIM-656766

Modern Language Association (MLA)

Faris, Bushra Hamzah…[et al.]. Molecular diagnosis of coccidioides posadasii from aborted placenta of ewes by RT-PCR. Kufa Journal for Veterinary Medical Sciences Vol. 5, no. 2 (2014), pp.24-31.
https://search.emarefa.net/detail/BIM-656766

American Medical Association (AMA)

Faris, Bushra Hamzah& Abd al-Majid, Ban Abbas& Majbil, Hamid Abd al-Husayn. Molecular diagnosis of coccidioides posadasii from aborted placenta of ewes by RT-PCR. Kufa Journal for Veterinary Medical Sciences. 2014. Vol. 5, no. 2, pp.24-31.
https://search.emarefa.net/detail/BIM-656766

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 30-31

Record ID

BIM-656766