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Molecular diagnosis of newcastle disease Iraqi virulent strain virus HN gene by specific primers design
Other Title(s)
التشخيص الجزيئي لمورث الهموكلوتنين نيوامنيديز للعترة المحلية الضارية لفيروس النيوكاسل في العراق باستخدام بادئات مصممة
Joint Authors
Hasan, Murtada Abd al-Mahdi Muhammad
al-Shammari, Ahmad Majid Hamzah
al-Nasrawi, Huda Abd al-Hadi Ali
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 5, Issue 2 (31 Dec. 2014), pp.196-203, 8 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2014-12-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry, The first report in which NDV was consider as zoonotic disease and a human pathogen that was published by Burnet, in 1943.
The aims of this study was to detect HN gene of two NDV Iraqi isolates (Najaf APMV1/ Chicken/ Iraq-najaf/ ICCMGR/2012and Baghdad ICCMGR) by designing specific priemrs for the Iraqi isolates.
DNV isolates propagated in embryonated chicken eggs and showed to kill embryos in different times intervals ( more than 40 hours and less than 72 hours) with marked sever hemorrhage in infected embryos and it was similar in the two isolates, agglutination activity reflect titer (1024) for Najaf isolate and (512) for Baghdad isolate.
The present study successfully detected part of (HN) gene by using specific primers designed for one-step RT-PCR undertaken to use them on molecular diagnosis of the hemagglutinin-neuraminidase (HN) gene in the Iraqi isolates and can be used for NDV virus detection in Iraq.
American Psychological Association (APA)
al-Shammari, Ahmad Majid Hamzah& al-Nasrawi, Huda Abd al-Hadi Ali& Hasan, Murtada Abd al-Mahdi Muhammad. 2014. Molecular diagnosis of newcastle disease Iraqi virulent strain virus HN gene by specific primers design. Kufa Journal for Veterinary Medical Sciences،Vol. 5, no. 2, pp.196-203.
https://search.emarefa.net/detail/BIM-656929
Modern Language Association (MLA)
al-Shammari, Ahmad Majid Hamzah…[et al.]. Molecular diagnosis of newcastle disease Iraqi virulent strain virus HN gene by specific primers design. Kufa Journal for Veterinary Medical Sciences Vol. 5, no. 2 (2014), pp.196-203.
https://search.emarefa.net/detail/BIM-656929
American Medical Association (AMA)
al-Shammari, Ahmad Majid Hamzah& al-Nasrawi, Huda Abd al-Hadi Ali& Hasan, Murtada Abd al-Mahdi Muhammad. Molecular diagnosis of newcastle disease Iraqi virulent strain virus HN gene by specific primers design. Kufa Journal for Veterinary Medical Sciences. 2014. Vol. 5, no. 2, pp.196-203.
https://search.emarefa.net/detail/BIM-656929
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 201-203
Record ID
BIM-656929