Seropositivity to capripoxvirusof sample collected from goats, cows and buffaloes

Abstract EN

In this study indirect ELISA test was conducted on 580 serum samples collected from 244 goats, 146 cows and 190 buffaloes.

Indirect ELISA was applied on all these samples depending on the optical density (OD) value (3mean +3OD) of sera previously prepared from blood of well know healthy and non-vaccinated goats, cows, buffaloes.

One negativity cut-off was selected for each animal sera as follows, goat(0.056), cow (0.083), and (0.104) in case of buffaloes sera.

there are (45%) of tested animal serum samples were seropositivity.

In concern of animal species, the higher overall seropositivity ratio was observed in sera of cows (50%), goats (40.2%) and buffaloes (47.4%).According to animals sex; in goats there were identical ratio of seropositivity (40%) and (40.2%) in both male and female respectively, while in cows and buffaloes; females showed higher ratio of seropositivity (57.7 and 50%) respectively.

According to age of animal the higher ratio are (41.1%, 57.3% and 50.3% respectively) was observed in first age group.

a significant differences were observed in the seropositivity concerning to age groups and sex of cow, while no significant differences were observed for goats and buffaloes.

According to the place of studied animals; the higher ratio of seropositivity were found in goats, cows and buffaloes (41.5%, 53.6% and 52.6%) respectively was observed in Thi-Qar province and then in Basrah province (5.9%) in goats, (42.9%) in cow and (42.1%) in buffaloes.

Depending on the period of the study, there was significant difference in the rate of seropositivity against Capripoxvirus.

The higher rate of seropositivity was observed in the sera which collected in February (53.6%) followed by March (49.1%).