Investigation of the effect of different concentrations of sildenafil Citrate (viagra)‎™ on sperm parameters during in vitro human Sperm activation in asthenozoospermic patients

Abstract EN

This study was designed to investigate the effect of different concentration of sildenafil citrate on sperm parameters during in vitro human sperm activation in asthenozoospermic patients.

Forty five infertile patients were involved in this study.

Modified Earl's medium (MEM) was used only as a control group (G1:15 patients) and two treated group with different concentrations of sildenafil citrate (G2: 15 patients; MEM supplemented with 100 ngm/ml) and (G3: 15 patients; MEM supplemented with 200 ngm/ml) for in vitro sperm processing.

spermatozoa were prepared by using direct layering technique.

Furthermore, sperm concentration, sperm motility, progressive sperm motility, sperm agglutination, and normal sperm morphology were evaluated according to standard WHO criteria (1999).

For preparation technique, sperm prepared and incubated for 30 minute in 5% CO2 at 37C after sperm processing.

The Results of the present study indicated a significant differences (P<0.05) in sperm motility (%) for in vitro postpreparation when compared with the control group.

In addition, a lower concentration (100 ngm/ml) of sildenafil citrate showed a significant enhancement in progressive sperm motility as compared with the control group.

It was concluded that a low dose of sildenafil citrate concentration give the best results in sperm preparation by increase motility and progressive motility.

Also, further studies are recommended to assess the effect of sildenafil citrate on in vitro assisted reproductive technologies results (ART) and intrauterine insemination (IUI).