Reverse-FIA technique for the determination of omeprazole using chemiluminescence detection

Abstract EN

Simple, accurate, sensitive and reliable reverse flow injection analysis (rFIA) technique with chemiluminescence detection was applied for the determination of omeprazole in pharmaceutical products.

This rFIA system was based on the inhibition effect of omeprazole on the generated chemiluminescence light from the in situ peroxynitrite (NO2 and H2O2 in acidic medium) and luminol system, when the latter was injected as a reagent.

All analytical parameters participated in the determination procedure were evaluated statistically to assess the applications of the methods.

Calibration curve was constructed between different omeprazole concentrations (μg/mL) versus the differences in CL-intensity in the form of the peak height (ΔCL = CL1 (Blank) - CL2 (omeprazole)).

The calibration curve was linear in concentration range of 3.0-15.0 μg/mL (R2= 0.9987 and DL= 1.30 μg/mL).

The method shows good selectivity as no serious effects of interferences were observed.

Applications of the present method for the determination of omeprazole in pharmaceutical products were performed and the results show good agreement with that obtained from HPLC technique.