Adhesion molecules expression in CLL : potential impact on clinical and hematological parameters

Abstract EN

Background : B-cell chronic lymphocytic leukemia (CLL) is marked by the accumulation of CD5+ B lymphocytes within the blood, bone marrow (BM), and secondary lymphoid tissues.

Abnormalities in the expression and function of cell adhesion molecules may account for the patterns of intra-nodal growth and hematogenous spread of the malignant cells.

Chemokines and integrin-mediated adhesion and trans-endothelial migration (TEM) are central aspects in trafficking and retention of hematopoietic cells in the BM and lymphoid organs.

Aim of the work : This work was conducted to study adhesion molecules status in CLL and its potential impact on both hematological and clinical parameters.

Patients and methods: The study included 78 newly diagnosed CLL patients.

Immunophenotyping was performed on peripheral blood using the chronic lymphoid panel.

Adhesion molecules (CD11a, CD11b, CD49d, CD49C, CD29 and CD38) were tested using monoclonal antibodies and analyzed by Flow Cytometry.

Results : Positive correlation was encountered between adhesion molecules: CD38 with CD49d (r = 0.25, p = 0.028), CD11a with CD11b, CD49d and CD29 (r = 0.394, p = 0.001; r = 0.441, p = < 0.01 and r = 0.446, p < 0.01 respectively) and CD29 with CD49c and CD49d (r = 0.437, p< 0.01; r = 0.674, p< 0.01 respectively).

CD49c showed negative correlation with Rai staging (r = 0.269, p =.033).

CD11a and CD29 showed a significant relation with splenomegaly (p = 0.04 and 0.03 respectively) and CD49d showed a significant relation with lymphadenopathy (p = 0.02).

Conclusion : The level of different adhesion molecules expression in CLL is apparently reflected on the potential migratory behavior of the leukemic cells to different organs