Gastroprotective activity of Eruca sativa leaf extract on ethanol-induced gastric mucosal injury in rattus Norvegicus

Abstract EN

Eruca sativa (Es), known as jarjeer, have been used in traditional medicine for the treatment of different diseases.

First, its powder was subjected to Energy-Dispersive x-Ray Fluorescence Analysis to determine the mineral content.

Then the plant was extracted by 95% of ethanol to evaluate anti-ulcerogenic activity against ethanol induced gastric ulcer.

For this purpose, thirty rats were divided into 6 groups n=5.

Respectively, all the animals were orally pre-treated with water, 10% Tween 20, omeprazole 20 mg/kg, 250, 500 and 750 mg/kg plant extract one hour before treating with absolute ethanol to generate gastric mucosal injury.

After additional hour, rats were anaesthetized and sacrificed; the gastric content was then collected and stomachs were examined to determine mucosal lesions.

The results showed that Es contains several beneficial minerals in which potassium showed to be in highest content 22.02±1.2%.

Grossly and histologically, the Tween 20 group exhibited severe mucosal injury, whereas the pre-treated rats with plant extract exhibited a significant protection in dose dependent manner.

Further, Es caused elevation of pH of gastric content and mucus production.

Therefore, it can be concluded that Es-ethanol leaf extract exhibits an anti-ulcer activity against ethanol-induction model through maintaining the acid base balance of gastric content.