Expression analysis of ClpB Hsp100 gene in faba bean (Vicia faba L.)‎ plants in response to heat stress

Abstract EN

Heat stress adversely affects the growth and yield of faba bean crop.

Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants.

Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species.

Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains.

ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thaliana cytoplasmic Hsp101 antibodies.

The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress.