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Assaying telomerase activity in three different malignant conditions in egyptian patients employing the polymerase chain reaction technique
Joint Authors
Mansur, Muhammad Tariq Ali
Abd al-Fattah, Imad Ibrahim
Samir Muhammad Abd Al-Munim
Ismail, Imad Al-Din Fuad
al-Sirafi, Tahir Ibrahim
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 14, Issue 3 (30 Sep. 2002), pp.229-236, 8 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2002-09-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Background : telomerase enzyme adds hexameric repeats of ‘5-TTAGGG-3’ to the end of mammalian chromosomal DNA (telomeres) to compensate for the progressive loss that occurs with successive rounds of DNA replication.
Significant telomerase activity has been identified in human germline cells in neoplastic immortal somatic cells, in normal human lymphocytes and in stem cells, but not in most normal somatic cells, which senesce after a certain number of cell divisions.
Objective : the present study was conducted to examine telomerase activity in three different common malignant conditions in Egypt : 1) treated and untreated chronic myeloid leukemia (CML), 2) hepatocellular carcinoma (HCC) with and without type B or C viral hepatitis and 3) urinary bladder carcinoma (UBC) with and without bilhar-ziasis.
Material and Methods : in CML, twenty subjects were included in each group and results were compared to a control group of age-matched apparently normal individuals.
In HCC and UBC specimens were included in each group.
In CML cases, peripheral blood mononuclear cells were analyzed, while in HCC and UBC cases, tumor biopsy specimens were used.
Telomerase activity was estimated by polymerase chain reaction (PCR) followed by ELI-SA technique, which is a modification of the original telomerase repeat amplification protocol (TRAP).
This technique allows highly specific amplification of telome-rase-mediated elongation products combined with non-radioactive detection following an ELISA protocol.
Results: In CML, 85 % of untreated patients were te-lomerase positive which was significantly higher than treated patients (65 %) or control group (30 %).
In HCC, 57 % of cases were telomerase positive.
A significantly higher percentage of telomerase positive specimens were found among HCC cases associated with either HCV (75 %) or HBV (71.4 %) infection than in HCC cases alone (22.2 %).
In UBC, 69 % of patients were telomerase positive without differences between bilharzial and non-bilharzial cases.
Conclusion : elevated telomerase enzyme activity is associated with untreated or viral- complicated malignancies and can be used as an adjuvant to parameters used for monitoring tumor progression or the effect of therapy.
American Psychological Association (APA)
Abd al-Fattah, Imad Ibrahim& Samir Muhammad Abd Al-Munim& Ismail, Imad Al-Din Fuad& Mansur, Muhammad Tariq Ali& al-Sirafi, Tahir Ibrahim. 2002. Assaying telomerase activity in three different malignant conditions in egyptian patients employing the polymerase chain reaction technique. Journal of the Egyptian National Cancer Institute،Vol. 14, no. 3, pp.229-236.
https://search.emarefa.net/detail/BIM-67486
Modern Language Association (MLA)
Abd al-Fattah, Imad Ibrahim…[et al.]. Assaying telomerase activity in three different malignant conditions in egyptian patients employing the polymerase chain reaction technique. Journal of the Egyptian National Cancer Institute Vol. 14, no. 3 (Sep. 2002), pp.229-236.
https://search.emarefa.net/detail/BIM-67486
American Medical Association (AMA)
Abd al-Fattah, Imad Ibrahim& Samir Muhammad Abd Al-Munim& Ismail, Imad Al-Din Fuad& Mansur, Muhammad Tariq Ali& al-Sirafi, Tahir Ibrahim. Assaying telomerase activity in three different malignant conditions in egyptian patients employing the polymerase chain reaction technique. Journal of the Egyptian National Cancer Institute. 2002. Vol. 14, no. 3, pp.229-236.
https://search.emarefa.net/detail/BIM-67486
Data Type
Journal Articles
Language
English
Notes
includes biliographical references : p. 235-236
Record ID
BIM-67486