Immunological characterization of diphtheria toxin recovered from corynebacterium pseudotuberculosis
Joint Authors
Salim, Salah Abd al-Karim
Muhammad, Faridah Hasan
Husayn, Ashjan Muhammad
Musa, Ihab Muhammad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 2 (31 Mar. 2016), pp.282-287, 6 p.
Publisher
Publication Date
2016-03-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Abstract EN
Diphtheria toxin (DT) is a potent toxin produced by the so-called diphtheria group which includes Corynebacterium diphtheriae (C.
diphtheriae), Corynebacterium ulcerans (C.
ulcerans), and Corynebacterium pseudotuberculosis (C.
pseudotuberculosis).
The present investigation is aimed to study in detail the production of DT by C.
pseudotuberculosis.
Twenty isolates were obtained from sheep diseased with caseous lymphadenitis (CLA) and twenty-six isolates were obtained from 26 buffaloes diseased with oedematous skin disease (OSD).
All isolates were identified by standard microbiological and DT production was assayed serologically by modified Elek test and immunoblotting.
All sheep isolates were nitrate negative, failed to hydrolyze starch and could not produce DT, while all buffalo isolates (biotype II) revealed positive results and a specific band of 62 kDa, specific to DT, was resulted in all concentrated cell fractions (CF), but was absent from non-toxigenic biotype I isolates.
At the same time, another band of 31 kDa specific to the PLD gene was obtained with all isolates of biotype I and II.
Moreover, all isolates showed positive synergistic hemolytic activity and antagonistic hemolysis with b-hemolytic Staphylococci.
The obtained results also indicated that C.
pseudotuberculosis could be classified into two strains; non-toxigenic biotype I strain, which failed to produce DT as well as being negative to nitrate
American Psychological Association (APA)
Salim, Salah Abd al-Karim& Muhammad, Faridah Hasan& Husayn, Ashjan Muhammad& Musa, Ihab Muhammad. 2016. Immunological characterization of diphtheria toxin recovered from corynebacterium pseudotuberculosis. Saudi Journal of Biological Sciences،Vol. 23, no. 2, pp.282-287.
https://search.emarefa.net/detail/BIM-675080
Modern Language Association (MLA)
Salim, Salah Abd al-Karim…[et al.]. Immunological characterization of diphtheria toxin recovered from corynebacterium pseudotuberculosis. Saudi Journal of Biological Sciences Vol. 23, no. 2 (2016), pp.282-287.
https://search.emarefa.net/detail/BIM-675080
American Medical Association (AMA)
Salim, Salah Abd al-Karim& Muhammad, Faridah Hasan& Husayn, Ashjan Muhammad& Musa, Ihab Muhammad. Immunological characterization of diphtheria toxin recovered from corynebacterium pseudotuberculosis. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 2, pp.282-287.
https://search.emarefa.net/detail/BIM-675080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 286-287
Record ID
BIM-675080