Spectrophotometric determination of diclofenac sodium using 2, 4-dinitrophenylhydrazine in pure form and pharmaceutical preparations
Other Title(s)
التقدير الطيفي لديكلوفيناك الصوديوم باستعمال 4،2- ثنائي نايترو فنيل ھيدرازين بشكله النقي و بمستحضراته الصيدلانية
Joint Authors
Dikran, Sarmad Bahjat
Mahmud, Ru'ua Muayyid
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 28, Issue 3 (31 Dec. 2015), pp.129-141, 13 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract EN
A simple and sensitive spectrophotometric method is described for the determination of diclofenac sodium (DCL), in pure form and pharmaceutical formulations.
The method is based on the oxidation of 2,4-dinitrophenylhydrazine (2,4-DNPH) and coupling of the oxidized product with DCL in alkaline medium to give intensively colored chromogen which exhibits maximum absorption (λmax) at 600 nm, and the concentration of DCL was determined spectrophotometrically.
The optimum reaction conditions and other analytical parameters were evaluated.
In addition to classical univariate optimization, modified simplex method (MSM) has been applied in optimization of the variables affecting the color producing reaction.
Beer’s law is obeyed in the concentration range of 0.5-40 μg.mL-1 with molar absorptivity of 2.0994×104 L.mol-1.cm-1.
The limit of detection was found to be 0.020 μg.mL-1 and the Sandellʼs sensitivity value was 0.0152 μg.cm-2.
The proposed method was successfully applied to the determination of DCL in pharmaceutical preparations.
American Psychological Association (APA)
Dikran, Sarmad Bahjat& Mahmud, Ru'ua Muayyid. 2015. Spectrophotometric determination of diclofenac sodium using 2, 4-dinitrophenylhydrazine in pure form and pharmaceutical preparations. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 28, no. 3, pp.129-141.
https://search.emarefa.net/detail/BIM-679127
Modern Language Association (MLA)
Dikran, Sarmad Bahjat& Mahmud, Ru'ua Muayyid. Spectrophotometric determination of diclofenac sodium using 2, 4-dinitrophenylhydrazine in pure form and pharmaceutical preparations. Ibn al-Haitham Journal for Pure and Applied Science Vol. 28, no. 3 (2015), pp.129-141.
https://search.emarefa.net/detail/BIM-679127
American Medical Association (AMA)
Dikran, Sarmad Bahjat& Mahmud, Ru'ua Muayyid. Spectrophotometric determination of diclofenac sodium using 2, 4-dinitrophenylhydrazine in pure form and pharmaceutical preparations. Ibn al-Haitham Journal for Pure and Applied Science. 2015. Vol. 28, no. 3, pp.129-141.
https://search.emarefa.net/detail/BIM-679127
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 137-140
Record ID
BIM-679127