Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the teaching hospital of Kashan, Iran
Joint Authors
Amiri, Atena
Firuzih, Fayruzih
Muniri, Radwan
Zibai, Muhammad
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 18, Issue 3 (31 Mar. 2016), pp.1-7, 7 p.
Publisher
Publication Date
2016-03-31
Country of Publication
United Arab Emirates
No. of Pages
7
Main Subjects
Abstract EN
-Background: Extended-spectrum β-lactamases (ESBLs) is one of the most important mechanisms of resistance to β-lactams especially among Enterobacteriaceae family including Klebsiella spp.
Different types of extended-spectrum β-lactamases including CTX-M-type and PER enzymes are identified among gram negative bacteria.
Objectives: The current study aimed to determine the prevalence of CTX-M-type and PER extended-spectrum β-lactamases among Klebsiella spp.
isolated from clinical specimens in the teaching hospital of Kashan, Iran.
Patients and Methods: One hundred Klebsiella spp.
were isolated from clinical specimens of hospitalized patients at Shahid-Beheshti hospital from December 2012 to November 2013.
Disk diffusion method was used to determine the susceptibility of these isolates to 14 different antimicrobial agents; disks were purchased from MAST company (United Kingdom).
The phenotypic double disk synergy confirmatory test was used to screen the isolates to produce extended-spectrum β-lactamase.
DNAs of isolates were extracted using boiling method and PCR assay was used to characterize the blaCTX-M type and blaPER genes.
The purified PCR products were sent to Macrogen research company (Korea) for sequencing.
Results: Of the total 100 Klebsiella isolates, %93 was susceptible to imipenem.
Resistance to ampicillin, ceftazidime, ceftriaxone, aztreonam and cefotaxime was (92%), (67%), (65%), (64%) and (59%), respectively.
The phenotypic confirmatory test (PCT) confirmed that 35% (n = 35) of the isolates were ESBL-producing Klebsiella strains.
The prevalence of blaCTX-M type and blaRER genes among Klebsiella isolates were 28% (n = 28) and 9% (n = 9), respectively.
Conclusions: The prevalence of ESBL-producing Klebsiella strains in Shahid-Beheshti hospital in Kashan has increased.
The study concluded that there was a high prevalence of the blaCTX-M type gene among ESBL positive isolates
American Psychological Association (APA)
Amiri, Atena& Firuzih, Fayruzih& Muniri, Radwan& Zibai, Muhammad. 2016. Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the teaching hospital of Kashan, Iran. Iranian Red Crescent Medical Journal،Vol. 18, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-679175
Modern Language Association (MLA)
Amiri, Atena…[et al.]. Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the teaching hospital of Kashan, Iran. Iranian Red Crescent Medical Journal Vol. 18, no. 3 (Mar. 2016), pp.1-7.
https://search.emarefa.net/detail/BIM-679175
American Medical Association (AMA)
Amiri, Atena& Firuzih, Fayruzih& Muniri, Radwan& Zibai, Muhammad. Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the teaching hospital of Kashan, Iran. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 3, pp.1-7.
https://search.emarefa.net/detail/BIM-679175
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6-7
Record ID
BIM-679175