Partial association of restriction polymorphism of the ligand binding domain of human androgen receptor in prostate cancer
Joint Authors
al-Barbari, Ahmad
Nabil, Amirah
Husayn, Muhammad
al-Jundi, Sad
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 17, Issue 2 (30 Apr. 2016), pp.223-228, 6 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2016-04-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background: Human androgen receptor (AR) functions as a steroid-hormone activated transcription factor.
The receptor binds to its ligand (testosterone or dihydrotestosterone) and is translocated to the nucleus to stimulate the transcription of androgen responsive genes.
Mutations in the ligand binding domain (LBD) impair the receptor activity and play a crucial role in the development and progression of prostate cancer (PCa).
Materials and methods: This work was designated to investigate the restriction integrity of the LBD and its association with benign prostatic hyperplasia (BPH) and prostate cancer.
Exons of this domain (exons: 4–8) were amplified from prostate tissue of BPH and PCa patients and the restriction polymorphism was investigated by SmlI, HphI and Tsp45I restriction enzymes in both BPH and PCa groups.
Results: Data revealed the integrity of exons 4–6 in both BPH and PCa patients.
Exons 7 and 8, however have kept their constitutional pattern only in BPH patients.
Hph1 site showed an abnormal restriction pattern in 40% and 26.7% of PCa patients.
Also, Tsp45I demonstrated restriction polymorphism in 20% and 13% of PCa patients.
Conclusion: Our results indicate that the loss of the restriction integrity in the C-terminal part (exons: 7 and 8) of the LBD is associated with the progression of benign prostatic hyperplasia to prostate cancer.
2015 The Authors.
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American Psychological Association (APA)
Husayn, Muhammad& al-Barbari, Ahmad& al-Jundi, Sad& Nabil, Amirah. 2016. Partial association of restriction polymorphism of the ligand binding domain of human androgen receptor in prostate cancer. The Egyptian Journal of Medical Human Genetics،Vol. 17, no. 2, pp.223-228.
https://search.emarefa.net/detail/BIM-679339
Modern Language Association (MLA)
Husayn, Muhammad…[et al.]. Partial association of restriction polymorphism of the ligand binding domain of human androgen receptor in prostate cancer. The Egyptian Journal of Medical Human Genetics Vol. 17, no. 2 (Apr. 2016), pp.223-228.
https://search.emarefa.net/detail/BIM-679339
American Medical Association (AMA)
Husayn, Muhammad& al-Barbari, Ahmad& al-Jundi, Sad& Nabil, Amirah. Partial association of restriction polymorphism of the ligand binding domain of human androgen receptor in prostate cancer. The Egyptian Journal of Medical Human Genetics. 2016. Vol. 17, no. 2, pp.223-228.
https://search.emarefa.net/detail/BIM-679339
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 228
Record ID
BIM-679339