Molecular detection and antimicrobial activity of enterococcus faecalis and enterococcus faecium isolated from urinary tract infection (UTI)‎ patients, animals and poultry

Abstract EN

Microbiological and polymerase chain reaction (PCR) methods was used to detect the occurrence of Enterococcus faecalis and Enterococcus faecium among 95 urine samples collected from patients suffering from urinary tract infection (UTI) recurrence admitted to outpatient clinics of private and governmental hospitals in Sohag city and 102 fecal and cloacal swabs collected from reared animals and / or poultry in some patient´s households.

The results revealed that E.

faecalis and E.

faecium was detected in 13 (13.7 %) patients, among them 11 patients were reared animals and / or poultry in their households, also isolated from 15 (14.7 %) out of 102 fecal and cloacal samples of animals and poultry reared in 11 patient´s households.

Referring to antimicrobial resistance and presence of esp gene among all enterococcal strains, E.

faecalis and E.

faecium isolates of both human and animals possess resistance to some antimicrobials with clinically importance for human therapy and esp gene was detected in 11 (84.65) out of 13 E.

faecalis and E.

faecium strains isolated from UTI patients and in 9 (60 %) out of 15 E.

faecalis and E.

faecium strains isolated from the reared animals and poultry.

This study suggests that reared animals and poultry, miss use of antimicrobials and presence of esp gene considered a risk factors for UTI recurrence caused by enterococci in human