Molecular detection of human papillomavirus genotype-33 in tissues from patients with prostate cancer and benign prostatic hyperplasia

Abstract EN

Background : High oncogenic –risk genotypes of Human Papillomavirus (HPV) are a group of genetically related organisms that infect a wide range of human cells including prostate epithelium and induce proliferative changes in these cells that result in both benign and malignant tumors.

Objectives : This study aimed to detect DNA of HPV genotype-33 using in situ hybridization technique in prostatic tissues from benign prostatic hyperplasia and prostatic adenocarcinomas, and elucidate the association between these HPV genotypes and prostatic carcinogenesis.

Patients and methods: Fifty(50) formalin-fixed, paraffin embedded prostatic tissue blocks were obtained ,among them (25) tissue biopsies from prostatic carcinoma with different grades and (15) benign prostate hyper plastic tissue blocks as well as (10) apparently normal prostate tissue autopsies which were collected from the archives of Forensic Medicine Institute / Baghdad and used as prostate healthy control groups.

Detection and genotyping of HPV was done by highly sensitive in situ hybridization technique.

Results : Signals of in situ hybridization reactions that indicating presence of HPV-33 in prostate cancer tissues were detected in32% (12 out of 40 cases) whereas in BPH, HPV-33 was detected in 13 %( 2 out of 15 cases).

Non HPV-33 was detected in the apparently healthy control group .The highest percentage (37%) of positive- HPV33- DNA ISH reactions was found in tissues of prostatic carcinoma showing moderate differentiation.

Conclusion : Our results indicate that the oncogenic HPV-33 might contribute to the development of subset of prostate tumors.