Isolation and identification of actara 25 WG degrading bacteria from agricultural soils and evaluation of their activity

Abstract EN

Fifty five agricultural soil samples were collected from different sites in Hilla city.

These samples were screened for Actara 25 WG (Pesticide) degrading bacteria.

Pour plate method involving the use of serial dilutions was employed for the isolation of bacteria.

The isolated bacterial species were identified on the basis of morphological and biochemical characteristics.

The results explained that these isolates belonged to three different species including Pseudomonas aeruginose Aeromonas hydrophila and Psendomonas putida.

The activity of these isolates for pesticide degradation was evaluated due to increasing of viable count (CFU/ ml) , decreasing of pH value, and increasing of optical density (0.D600).

The results revealed all of these isolates have an ability of biogegradation , but the more active one is P.

putida which increase the viable count to 89×108(CFU/ ml) and the 0.D600 to (1.7) during the incubation period ( 7 days).

So the present study demonstrated that the isolated bacterial species could be harnessed to use in bioremediation of agricultural lands polluted with pesticide .