A microbial profiling method for the human microbiota using high-throughput sequencing
Joint Authors
Li, Fei
Tseng, Huei-Hun Elizabeth
Hullar, Meredith A. J.
Lampe, Johanna W.
Sandstrom, Richard
Johnson, Audra K.
Ruzzo, Walter L.
Stamatoyannopoulos, John
Source
Issue
Vol. 2, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Publication Date
2013-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Study of the human microbiota in relation to human health and disease is a rapidly expanding field.
To fully understand this complex relationship using low-coverage Illumina short reads, we develop a profiling method, microbial nucleotide signatures (MNSs), to characterize the human microbiota.
By calculating the nucleotide diversities along the sequenced 16S rRNA gene region, which did not require assembly or phylogenetic identification, we were able to differentiate the gut MNSs of 9 healthy individuals.
Applying MNSs to a public dataset showed that it could differentiate body site differences.
The scalability of our approach offers a rapid classification of study participants for studies with the sample sizes required for epidemiological studies.
Using MNSs to classify the microbiome associated with a disease state followed by a targeted in-depth sequencing will give a comprehensive understanding of the role of the microbiome in human health.
American Psychological Association (APA)
Tseng, Huei-Hun Elizabeth& Hullar, Meredith A. J.& Li, Fei& Lampe, Johanna W.& Sandstrom, Richard& Johnson, Audra K.…[et al.]. 2013. A microbial profiling method for the human microbiota using high-throughput sequencing. Metagenomics،Vol. 2, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-686088
Modern Language Association (MLA)
Li, Fei…[et al.]. A microbial profiling method for the human microbiota using high-throughput sequencing. Metagenomics Vol. 2 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-686088
American Medical Association (AMA)
Tseng, Huei-Hun Elizabeth& Hullar, Meredith A. J.& Li, Fei& Lampe, Johanna W.& Sandstrom, Richard& Johnson, Audra K.…[et al.]. A microbial profiling method for the human microbiota using high-throughput sequencing. Metagenomics. 2013. Vol. 2, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-686088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-8
Record ID
BIM-686088