Epstein barr virus in head and neck extranodal non-hodgkin’s lymphoma in Egypt
Joint Authors
al-Husayni, Sumayyah
Sidqi, Lobna
Khalid, Husayn M.
Mukhtar, Nadiyah M.
Bahnasi, Abir A.
Zikri, Abd al-Rahman N.
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 15, Issue 4 (31 Dec. 2003), pp.349-362, 14 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2003-12-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Topics
Abstract EN
Background : primary extranodal lymphoma of the head and neck (PELHN) represents 16.18 % of all malignant lymphoma cases diagnosed at the NCI, Cairo University between 1985-1990.
Epstein Barr virus (EBV) infection is highly associated with lymphoma in immune-compromised patients however ; the situation in immune-competent patients is still unclear.
Material and methods : we investigated 50 PELHN, 5 reactive lymph nodes, 15 normal nasopharyngeal tissue (NPTs) and 25 throat washes (TW) of healthy people from Egypt for EBV association, expression of p53, Ki-67, bcl-2, Box and the clinical outcome.
Results : phenotypic analysis showed that 31 cases were B cell and 19 were T cell lymphomas.
Eleven cases were present in the nose and Para nasal sinuses, 11 in the nasopharynx, 13 in the tonsils, 7 in the oropharynx and 8 in the oral cavity.
EBV positivity was reported in 90 % and 70 % of the cases using EBER in situ hybridization and PCR respectively (concordance = 70 %).
All cases of nasal type lymphoma were EBV positive.
Strain typing revealed the presence of type 1 in all positive cases.
30 base pair deletion of the LMP-1 (del-LMP1) gene was detected in 24 / 35 EBV-positive cases by PCR (68.6 %) whereas 11 cases had wild type variant either alone or mixed with del-LMP1.
There was a statistically significant difference in the frequency of del-LMP1 between lympho-ma and normal tissues.
Overexpression of Ki-67, p53 and bcl-2 was detected in 78.1 %, 62.5 % and 20 % of cases whereas absence of Box was reported in 18 % of cases.
Univar ate analysis showed that p53 overexpression, a high Ki-67 index ; advanced disease stage and T phenotype are poor prognostic factors whereas only p53 overexpression and advanced disease stage are poor prognostic factors in multivariate analysis.
Conclusion : EBV infection is frequent in PELHN in Egypt.
Possible pathogen etic mechanisms involve deregulation of p53 and enhanced proliferation (as detected by high Ki-67 index).
The presence of del-LMP1 variants, p53 overexpression and advanced disease stage are poor prognostic factors associated with reduced survival and poor response to therapy.
American Psychological Association (APA)
Bahnasi, Abir A.& Zikri, Abd al-Rahman N.& al-Husayni, Sumayyah& Khalid, Husayn M.& Sidqi, Lobna& Mukhtar, Nadiyah M.. 2003. Epstein barr virus in head and neck extranodal non-hodgkin’s lymphoma in Egypt. Journal of the Egyptian National Cancer Institute،Vol. 15, no. 4, pp.349-362.
https://search.emarefa.net/detail/BIM-68620
Modern Language Association (MLA)
Khalid, Husayn M.…[et al.]. Epstein barr virus in head and neck extranodal non-hodgkin’s lymphoma in Egypt. Journal of the Egyptian National Cancer Institute Vol. 15, no. 4 (Dec. 2003), pp.349-362.
https://search.emarefa.net/detail/BIM-68620
American Medical Association (AMA)
Bahnasi, Abir A.& Zikri, Abd al-Rahman N.& al-Husayni, Sumayyah& Khalid, Husayn M.& Sidqi, Lobna& Mukhtar, Nadiyah M.. Epstein barr virus in head and neck extranodal non-hodgkin’s lymphoma in Egypt. Journal of the Egyptian National Cancer Institute. 2003. Vol. 15, no. 4, pp.349-362.
https://search.emarefa.net/detail/BIM-68620
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 361-362
Record ID
BIM-68620