Bioethanol production by immobilized Saccharomyces Cerevisiae using different lignocellulesic materials

Other Title(s)

إنتاج الإيثانول الحيوي من بعض المخلفات الزراعية باستخدام خميرة الخباز بنظام التثبيت

Dissertant

al-Kafarnah, Afaf Dhib Abd al-Rahman

Thesis advisor

al-Bashiti, Tariq Abd al-Qadir
al-Kahlut, Kamal al-Abd Muhammad

Comitee Members

al-Qishawi, Abbud Yasir
al-Lahham, Nahid Ali

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2015

English Abstract

Ethanol from biomass is an attractive and sustainable energy source for transportation fuel to substitute gasoline.

Second generation ethanol production utilizes cheaper and non-food feed stocks like lignocelluloses or municipal solid waste, could make ethanol more competitive to fossil fuels.

Basically, most of the raw materials used for the production of bioethanol were corn grain and sugar cane.

However, it is also important to see the potential of the other agricultural raw materials rich in fermentable carbohydrates such as tomato wastes and wheat straw since it is available in Palestine and cheaper compared to the others.

The aim of the present study is the production of low cost cellulosic ethanol using basically the agro wastes like tomato waste and wheat straw and make a comparison between the efficiency of free and immobilized yeast cells in calcium alginate matrix with microwave-assisted acidic pretreatment.

In this study we have investigate the efficiency of immobilization technique for bioethanol production using Saccharomyces cerevisiae strain which isolated from yogurt.

This strain was identified according to morphological and biochemical characterization tests.

Microwave-assisted acidic pretreatment were performed for both wheat straw and tomato waste and show high improvement reach to 45% in reducing sugar amount compared with convection mode of heating of dilute 7%HCl and 5% H2SO4 hydrolysis.

Calcium alginate was used as immobilization matrix for S.

cerevisiae.

The best calcium alginate concentration was 3% and 4 % for reference and isolated yeast respectively.

The immobilization technique gave higher ethanol yield compared with free system for tomato waste but lower yield with wheat straw.

The maximum amount of ethanol (641mg/g) produced by free cells when used straw pretreated with microwave-assisted 5%H2SO4 hydrolysis and (543.51 mg/g) for tomato waste using immobilized cells with microwave-assisted 7%HCl hydrolysis.

Main Subjects

Biology

No. of Pages

68

Table of Contents

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Introduction.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Results.

Chapter Five : Discussion.

Chapter Six : Conclusion and recommendation.

References.

American Psychological Association (APA)

al-Kafarnah, Afaf Dhib Abd al-Rahman. (2015). Bioethanol production by immobilized Saccharomyces Cerevisiae using different lignocellulesic materials. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-687715

Modern Language Association (MLA)

al-Kafarnah, Afaf Dhib Abd al-Rahman. Bioethanol production by immobilized Saccharomyces Cerevisiae using different lignocellulesic materials. (Master's theses Theses and Dissertations Master). Islamic University. (2015).
https://search.emarefa.net/detail/BIM-687715

American Medical Association (AMA)

al-Kafarnah, Afaf Dhib Abd al-Rahman. (2015). Bioethanol production by immobilized Saccharomyces Cerevisiae using different lignocellulesic materials. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-687715

Language

English

Data Type

Arab Theses

Record ID

BIM-687715