Evaluation of plasma soluble human leukocyte antigen-G as a tumor marker for breast cancer patients in Gaza Strip

Other Title(s)

تقييم مستضد كريات الدم البيضاء البشرية القابلة للذوبان في البلازما من نوع G كعلامة أورام لمريضات سرطان الثدي في قطاع غزة

Dissertant

Khattab, Berta Ahmad Ubayd Allah

Thesis advisor

Yasin, Majid Muhammad
al-Yaziji, Mansur Subhi

Comitee Members

al-Uwayni, Saib Husayn
al-Lahham, Nahid Ali

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2015

English Abstract

-Background: Soluble Human leukocyte antigen-G (sHLA-G) has been closely associated with diagnosis and prognosis in many types of human cancer.

The current study aims to investigate whether or not plasma s HLA-G can be used as a potential biomarker for detection and diagnosis of breast cancer in Gaza strip.

Patients and Methods: The study is a case control and carried out in Al-Shifa hospital in Gaza city.

sHLA-G plasma levels were determined using a specific sandwich enzyme linked immunosorbent assay (ELISA) on 180 women aged 25-75 years.

The study population were divided into two groups; the (case group) consisted of 60 women with breast cancer and receiving anti-cancer treatment (post ) and 60 women who have breast cancer but did not receive chemotherapy (pre), and (control group) consisted of 60 healthy women.

CA15-3 and CEA levels were assayed by using the Axsym Immunoassay system.

SPSS system was used to analyze the data.

Results: Plasma sHLA-G levels were significantly higher in breast cancer patients compared to healthy controls (P<0.001), the Receiver-Operating Characteristic (ROC) curve of sHLA-G for discriminating patients with breast cancer (n= 120) from the control group (n= 60) was 0.919 (95% CI = 0.882 - 0.956), and was smaller than those of CA15-3 (0.998, 95% CI = 0.996 -1.00) and CEA (0.985, 95% CI = 0.973 - 0.997).

There were significant differences among the Area Under-ROCs(AUROCs) of sHLA-G, CA15-3, and CEA (P=0.000; sHLA-G vs.

CA15-3, P =0.000; sHLA-G vs.

CEA, P = 0.000; CA15-3 vs.

CEA, P = 0.000).

The cutoff values to differentiate the breast cancer group from the control group were 107 u/ml.

The AUROCs of sHLA-G, CEA, and CA15-3 for differentiating the group of women who did not receive chemotherapy yet (n = 60) and the group of women who received anti-cancer treatment (n = 60) were 0.998,0.781 and 0.698, respectively(P< 0.001), the cutoff values to differentiate between the two groups were 216 u/ml, 4.2 ng/ml, and 42.45 u/ml, respectively.

We also investigate the correlation of sHLA-G with CA15-3 and CEA, the levels of sHLA-G positive significantly correlate with CEA and CA15-3 (r =0.701, P< 0.001 and r =0.666, P< 0.001, respectively) in the total study population.

For the correlation of sHLA-G with Hb, the results showed good positive correlations according to Spearman’s roh test.

In addition, we found statistically significant differences at 0.05 in sHLA-G, CEA, CA15-3, Hb and WBC between the pre group and post group ( U= 2.00, 179.0, 271.5, 313.50, and 29.00 respectively, p< 0.001).

Furthermore, 53 % of breast cancer women were younger than 55 years old.

Conclusion: Our study may provide evidence in further support of the application of sHLA-G as a biomarker in body fluids for breast cancer diagnosis and monitoring of breast cancer therapy.

Main Subjects

Medicine

No. of Pages

93

Table of Contents

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Language

English

Data Type

Arab Theses

Record ID

BIM-688483