Cloning, expression and purification of putative isovaleryl-CoA dehydrogenase from Paracoccus denitrificans Pd1222
Other Title(s)
استنسال و تعبير و تنقية الانزيم المفترض CoA dehydrogenase من بكتريا Paracoccus denitrificans Pd1222
Joint Authors
al-Azami, Abd al-Karim Jasim Hashim
Karim, Rafid Muhammad
Source
Issue
Vol. 57, Issue 2B (30 Jun. 2016), pp.1142-1149, 8 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
In Paracoccus denitrificans Pd1222 bacterium, Pden_3633 encoding gene has been nominated to encode for Isovaleryl CoA dehydrogenase (IVDH) [1], the enzyme which involve in leucine catabolism pathway.
In this study, this putative IVDH was investigated.
IVDH encoding gene from P.
denitrificans Pd1222 in addition to desired features for cloning, expression and purification have been designed and synthesized.
The synthetic coding sequence was expressed in Escherichia coli.
The enzyme was purified as a Strep-Tagged protein with a total protein 220.5 mg.
An apparent molecular weight of 42.9 kDa was determined on SDS gel.
Amino acid alignment showed a very high similarity (91-96%) with corresponding IVDH from several other Paracoccus species.
As for genera other than Paracoccus; Roseovarius mucosus, Catellibacterium nectariphilum and Oceanicola nanhaiensis recorded the highest similarity (85-86%), Suggesting that these mentioned species all have similar IVDH.
American Psychological Association (APA)
Karim, Rafid Muhammad& al-Azami, Abd al-Karim Jasim Hashim. 2016. Cloning, expression and purification of putative isovaleryl-CoA dehydrogenase from Paracoccus denitrificans Pd1222. Iraqi Journal of Science،Vol. 57, no. 2B, pp.1142-1149.
https://search.emarefa.net/detail/BIM-688646
Modern Language Association (MLA)
Karim, Rafid Muhammad& al-Azami, Abd al-Karim Jasim Hashim. Cloning, expression and purification of putative isovaleryl-CoA dehydrogenase from Paracoccus denitrificans Pd1222. Iraqi Journal of Science Vol. 57, no. 2B (2016), pp.1142-1149.
https://search.emarefa.net/detail/BIM-688646
American Medical Association (AMA)
Karim, Rafid Muhammad& al-Azami, Abd al-Karim Jasim Hashim. Cloning, expression and purification of putative isovaleryl-CoA dehydrogenase from Paracoccus denitrificans Pd1222. Iraqi Journal of Science. 2016. Vol. 57, no. 2B, pp.1142-1149.
https://search.emarefa.net/detail/BIM-688646
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-688646