Effect of long-term exposure to mobile phone radiation on alpha-int1 gene sequence of candida albicans

Abstract EN

Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated.

Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans.

A standard 900 MHz radiation generator was used for radiation.

10 ml volumes from a stock suspension of C.

albicans were transferred into 10 polystyrene tubes.

Five tubes were exposed at 4 C to a fixed magnitude of radiation with different time periods of 10, 70, 210, 350 and 490 h.

The other 5 tubes were kept far enough from radiation.

The samples underwent genomic DNA extraction.

PCR amplification of alpha-Int1 gene sequence was done using one set of primers.

PCR products were resolved using agarose gel electrophoresis and the nucleotide sequences were determined.

All samples showed a clear electrophoretic band around 441 bp and further sequencing revealed the amplified DNA segments are related to alpha-Int1 gene of the yeast.

No mutations in the gene were seen in radiation exposed samples.

Long-term exposure