Preparation of nanoliposomes by size exclusion chromatography to entrap soluble antigens from leishmania donovani
Other Title(s)
تحضير الجسيمات الدهنية النانوية باستخدام كروموتوكرافيا الترشيح الحجمي لاقتناص المستضدات الذائبة لطفيلي اللشمانيا دونفاني
Joint Authors
Abd Allah, Rawah Najm
al-Luhaybi, Raghad Kazim
al-Halbusi, Muhammad Mahmud Farhan
Taha, Ali Abd al-Rahman
Source
Issue
Vol. 57, Issue 2A (30 Jun. 2016), pp.814-823, 10 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
In this study, we investigated the ability of nanoliposomes preparation, as a nanoadjuvant, to entrap soluble Leismania donovani antigens (SLAs) and release in vitro.
The parasite reactivation was carried out when inoculated into Rosewell park memorial institute media (RPMI) and incubated at 23 °C for 4 days.
L.
donovani promastigote inoculum (104 cell / ml) of 4 days was used to inoculate modified medium of Saline - Neopeptone and Blood agar 9 (SNB 9) to produce promastigote mass.
SLAs were extracted from the promastigotes ghost membrane after fourth passages of subculturing in SNB.
The membrane pellet obtained was suspended in 5 mM Tris buffer (pH 7.6) and sonicated three times at 4 °C and entrapped in freshly prepared nanoliposomes.
Lipids mixture of 4mM Phosphatidylcholine, 2.2 mM Cholesterol and 0.55 mM Phosphatidylethanolamine in a ratio of 7:2:1 were used to prepare nanoliposome.
Physio-chemical characterizations of prepared nanoliposomes was performed by using Scanning Electron Microscope (SEM) , Atomic Force Microscope (AFM) and Zeta Potential assays to determine the size, morphology and charge.
The efficiency of freshly prepared nanoliposoms to entrap SLAs was determined by measuring the nanoliposome efficiency entrapment (EE).
The percentage of EE was 50 and 27.5 of SLAs entrapped nanoliposomes prepared by Sephadex G25 and Sephadex G75, respectively.
Moreover, stability of SLAs entrapped nanoliposomes was examined at 4 and 37 °C as a storage temperature
American Psychological Association (APA)
Abd Allah, Rawah Najm& al-Luhaybi, Raghad Kazim& al-Halbusi, Muhammad Mahmud Farhan& Taha, Ali Abd al-Rahman. 2016. Preparation of nanoliposomes by size exclusion chromatography to entrap soluble antigens from leishmania donovani. Iraqi Journal of Science،Vol. 57, no. 2A, pp.814-823.
https://search.emarefa.net/detail/BIM-692083
Modern Language Association (MLA)
Abd Allah, Rawah Najm…[et al.]. Preparation of nanoliposomes by size exclusion chromatography to entrap soluble antigens from leishmania donovani. Iraqi Journal of Science Vol. 57, no. 2A (2016), pp.814-823.
https://search.emarefa.net/detail/BIM-692083
American Medical Association (AMA)
Abd Allah, Rawah Najm& al-Luhaybi, Raghad Kazim& al-Halbusi, Muhammad Mahmud Farhan& Taha, Ali Abd al-Rahman. Preparation of nanoliposomes by size exclusion chromatography to entrap soluble antigens from leishmania donovani. Iraqi Journal of Science. 2016. Vol. 57, no. 2A, pp.814-823.
https://search.emarefa.net/detail/BIM-692083
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 822-823
Record ID
BIM-692083