Using of species-specific primers for molecular diagnosis of in vitro promastigotes of leishmania donovani
Other Title(s)
استخدام بادىء متخصص للتشخيص الجزيئي لطفيلي اللشمانيا الاحشائية للاطوار المسوطة خارج الجسم الحي
Joint Authors
Yasin, Farah Tariq
al-Samirrai, Haydar Zuhayr Ali
Source
Issue
Vol. 57, Issue 2A (30 Jun. 2016), pp.824-829, 6 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
Visceral Leishmaniasis (VL) is a disseminated protozoan infection caused by Leishmania donovani parasites which affects almost half a million persons annually.
Classical diagnosis methods of VL still not very sensitive and time consuming.
In this study, we reported the success of polymerase chain reaction (PCR) method to identify L.
donovani based on kinetoplast deoxyribonucleic acid (kDNA) for the diagnosis of the parasite using in vitro promastigote cultures.
LdI species - specific primer was used to identify L.
donovani and the result showed a single band of about ~600bp.
It can be recommended that this primer is to be used for detection the visceral L.
donovani.
American Psychological Association (APA)
Yasin, Farah Tariq& al-Samirrai, Haydar Zuhayr Ali. 2016. Using of species-specific primers for molecular diagnosis of in vitro promastigotes of leishmania donovani. Iraqi Journal of Science،Vol. 57, no. 2A, pp.824-829.
https://search.emarefa.net/detail/BIM-692110
Modern Language Association (MLA)
Yasin, Farah Tariq& al-Samirrai, Haydar Zuhayr Ali. Using of species-specific primers for molecular diagnosis of in vitro promastigotes of leishmania donovani. Iraqi Journal of Science Vol. 57, no. 2A (2016), pp.824-829.
https://search.emarefa.net/detail/BIM-692110
American Medical Association (AMA)
Yasin, Farah Tariq& al-Samirrai, Haydar Zuhayr Ali. Using of species-specific primers for molecular diagnosis of in vitro promastigotes of leishmania donovani. Iraqi Journal of Science. 2016. Vol. 57, no. 2A, pp.824-829.
https://search.emarefa.net/detail/BIM-692110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 827-829
Record ID
BIM-692110