Influence of some sugars on in vitro micropropagation of obione portulacoides (L.)‎ moq. explants on hormone-free media

Abstract EN

Using In vitro cultivated explants, of Obione portulacoïdes (L.) Moq was regenerated.

The Obione portulacoïdes (L.) Moq is a halophyte shrub widely distributed in temperate Eurasia and parts of Africa, found in salty marshes and coastal dunes.

This research was conducted to study, in particularly, the influence of three carbohydrate sources, i.e.

sucrose, fructose and xylose (essentially in the absence of growth regulators) on different morphogenic reactions.

In fact, there is a potential possibility for regenerating complete plants without any exogenous hormone.

The highest shoots number (4.2) and highest roots number (6.3) were observed when culturing on medium supplemented with a mixture of fructose-xylose, followed by (2.9) and (4.2) respectively in presence of xylose.

The observed dry weight was (9.3 mg) in presence of xylose, followed by (7.1 mg) in presence of the mixture (fructosexylose), and (5.4 mg) when sucrose is added.

The rooting was stopped in the presence of fructose.

These results open numerous perspectives concerning both a fundamental approach of morphogenesis in connection with tissue nutrition and a special applied interest for the media containing a chosen hydrocarbon source, and deprived of growth regulators- free, for conform multiplication.