Utilizing chemotherapy for efficient elimination of potato leaf roll virus and potato Y virus

Abstract EN

plant (indexed by ELISA and RT-PCR) were cultured on MS medium supplemented with NAA (0.1 mgl-1) containing Ribavirin (RBV) and Azacitidine (AZA) each at 0, 20, 40 and 60 mgl-1.

Results of RT-PCR procedure showed that RBV, at all applied concentrations, eliminated PVY and PLRV viruses from Chandramukhi infected vitroplants grown from sprout tips length 3 and 5 mm.

while PVY was destroyed by AZA in Chandramukhi infected vitroplants grown from length 3 and 5 mm sprout tips at concentrations of 20 and 40 mgl-1 respectively.

PLRV in the same cultivar was eradicated from infected vitroplants developed of sprout tip of 3 and 5 mm length at 60 mgl-1 of AZA and also from sprout tips of 3 mm length at 20 mgl-1.

Elimination efficiency of Ribavirin in eradicating PVY and PLRV viruses from Marfona infected vitroplants grown from sprout tips of both sizes (3 and 5 mm in length) could not have positive outcome as compared to Chandramukhi cultivar.

But those Marfona infected vitroplants treated with Azacitidine doses (20, 40 and 60 mgl-1) showed negative reaction with RT-PCR.

These results showed that the response of two potato cultivars to antivirals were different, and the importance of these results comes from its application value for producing virus free plants and propagating them by tissue culture technique.This research was carried out to study the efficiency of some antivirals for efficient elimination potato leaf roll virus and potato y virus.

In this investigate the segments of sprout tips (3.0 and 5.0 mm in length ) from infected mother