Analysis of DNA polymorphisms in Arabic camel (Camelus dromedarius) using random amplified polymorphic DNA polymerase chain reaction technique
Other Title(s)
تحليل التشكل الوراثي للحامض النووي الرايبوزي منقوص الأوكسجين في الإبل العربية (Camelus dromedarius) باستعمال تقنية التضخيم العشوائي متعدد الأشكال لقطع DNA
Joint Authors
Jayid, Talib Ahmad
Yusuf, Muontaha Yaqub
Muhsin, Iftikhar Hasan
Sakr, Dirgham Kamil
Source
Basrah Journal of Agrricultural Science
Issue
Vol. 26, Issue 1 (30 Jun. 2013), pp.60-68, 9 p.
Publisher
University of Basrah Agriculture College
Publication Date
2013-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract EN
Camel populations in Iraq are facing a severe decline which demands immediate actions to ensure its conservation.
This study was carried out at molecular genetics laboratory, college of agriculture, university of Basrah.
Twenty four blood samples were analyzed by random amplified polymorphic DNA (RAPD) to investigate genetic similarity and diversity among and within three Arabic camel flocks (Camelus dromedarius) represented in Basrah, Thy Qar and Muthanna provinces.
Ten random primers were used to amplify DNA fragments in these three flocks.
Among ten tested RAPD primers, 6 primers generated novel and polymorphic DNA fragments in all tested samples.
A total of 381 bands were obtained, 168 of them were polymorphic.
The number of polymorphic bands in each population was found to be different and was higher in the Muthanna than in Thy Qar and Basrah provinces (76, 50 and 42) bands, respectively.
Number of polymorphic bands in Muthanna province population was approximately 1.5 times as diverse as that in Basrah and Thy Qar provinces populations.
Comparison of banding patterns in Muthanna, Basrah and Thy Qar populations revealed substantial differences that the populations may have been subjected to a long period of geographical isolation from each other.
Relationship was determined on the base of polymorphic products analysis and presented in the form of dendrogram (UPGMA percent method).
There is a significant correlation between geographical distance and genetic distance between populations.
RAPD analysis confirmed the presence of genetic variation within tested Arabic camel.
The obtained data will help build conservation strategies to avoid the extinction of animals from their natural habitats in the future.
RAPD technique makes it ideal for genetic mapping, plant and animal breeding programs and DNA fingerprinting, with particular utility in the field of population genetics.
This study compared, for the first time, variation in DNA fingerprinting of Arabic camels in Iraq.
American Psychological Association (APA)
Jayid, Talib Ahmad& Yusuf, Muontaha Yaqub& Muhsin, Iftikhar Hasan& Sakr, Dirgham Kamil. 2013. Analysis of DNA polymorphisms in Arabic camel (Camelus dromedarius) using random amplified polymorphic DNA polymerase chain reaction technique. Basrah Journal of Agrricultural Science،Vol. 26, no. 1, pp.60-68.
https://search.emarefa.net/detail/BIM-695466
Modern Language Association (MLA)
Jayid, Talib Ahmad…[et al.]. Analysis of DNA polymorphisms in Arabic camel (Camelus dromedarius) using random amplified polymorphic DNA polymerase chain reaction technique. Basrah Journal of Agrricultural Science Vol. 26, no. 1 (2013), pp.60-68.
https://search.emarefa.net/detail/BIM-695466
American Medical Association (AMA)
Jayid, Talib Ahmad& Yusuf, Muontaha Yaqub& Muhsin, Iftikhar Hasan& Sakr, Dirgham Kamil. Analysis of DNA polymorphisms in Arabic camel (Camelus dromedarius) using random amplified polymorphic DNA polymerase chain reaction technique. Basrah Journal of Agrricultural Science. 2013. Vol. 26, no. 1, pp.60-68.
https://search.emarefa.net/detail/BIM-695466
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 67-68
Record ID
BIM-695466