Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique
Other Title(s)
التشخيص المباشر لطفيلي Entamoeba bovis في العجول المصابة بالإسهال باستخدام تقنية تفاعل سلسلة البلمرة
Author
Source
Kufa Journal for Veterinary Medical Sciences
Issue
Vol. 7, Issue 1 (30 Jun. 2016), pp.132-137, 6 p.
Publisher
University of Kufa Faculty of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
This study carried out to direct molecular investigation of Entamoeba bovis from feces samples of calves which suffering from diarrhea that collected from different fields in Al-Diwanyia city by using polymerase chain reaction technique (PCR).
This technique was dependent on used specific primers that amplification of small subunit ribosomal RNA gene in Entamoeba bovis.
This primers were designed in this study by using NCBI-Genk data base (FN666248.1) and primer 3 plus for primers design.
The PCR results were appeared that cattle infected with Entamoeba bovis in percentage of about (36% ) 18 positive samples out of 50 diarrheic samples.
We concluded that Entamoeba bovis is important causes of enteric infection in calf whereas, the polymerase chain reaction technique is very specific and rapid assay.
American Psychological Association (APA)
al-Shabani, Amal Hasan Abd. 2016. Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique. Kufa Journal for Veterinary Medical Sciences،Vol. 7, no. 1, pp.132-137.
https://search.emarefa.net/detail/BIM-695711
Modern Language Association (MLA)
al-Shabani, Amal Hasan Abd. Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique. Kufa Journal for Veterinary Medical Sciences Vol. 7, no. 1 (2016), pp.132-137.
https://search.emarefa.net/detail/BIM-695711
American Medical Association (AMA)
al-Shabani, Amal Hasan Abd. Direct detection of Entamoeba bovis in calves infected by diarrhea by using Polymerase chain reaction technique. Kufa Journal for Veterinary Medical Sciences. 2016. Vol. 7, no. 1, pp.132-137.
https://search.emarefa.net/detail/BIM-695711
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 135-137
Record ID
BIM-695711