The spectrophotometric determination of olanzapine via coupling with diazotized p-itroaniline

Abstract EN

A new spectrophotometric method has been developed for the assay of olanzapine (OLN.) in pure and dosage forms.

The method is based on the diazocoupling of (OLN.) with diazotized p-nitroaniline in alkaline medium to form a stable brown colored water-soluble azo dye with a maximum absorption at 405 nm.

The variables that affect the completion of reaction have been carefully optimized.

Beer’s law is obeyed over the concentration range of (0.5-45.0 μg.mL-1) with a molar absorptivity of 1.5777×104 L.mol-1.cm-1.

The limit of detection was 0.3148 μg.mL-1 and Sandell’s sensitivity value was 0.0198 μg.cm-2.

The proposed method has been applied successfully to the determination of olanzapine in tablet pharmaceutical preparations.