Effect of adding different levels of green tea infusion to semen extenders on some characteristics of the chilled-stored Awassi ram semen

Abstract EN

The aim of this study was to assess the effect of adding different levels of green tea (GT) infusion to semen extenders on the percentage of motility, viability and abnormality of ram semen during storage at 5◦C for 0, 24, 48, 72, 96, 120 and 144 hrs after collection and dilution.

A total of 16 ejaculates from 3 Awassi rams were gathered by artificial vagina and extended by TRIS extender.

The GT infusion was added by 0, 2, 4 and 6 ml/ 100 ml of extender which represents control group (C) and treatment groups (T1, T2 and T3) respectively.

The results demonstrated a significant increase (P<0.05) in the percentage of sperm motility for each of the treatments T2 and T3 as compared with control group (C) for periods 0, 24, 48, 72, 96 and 120 hrs, while the treatment T1 showed a calculation increased for the same trait as compared with the control group (C) for all periods of experiment.

The percentage of sperm viability showed a calculation increased for all treated groups T1, T2 and T3 as compared with control group (C) for all periods of experiment.

Otherwise, the results appeared a significant decrease (P<0.05) in the sperm abnormalities percentage for both treatments T2 and T3 as compared with the control group (C) for all periods of experiment, whereas the treatment T1 revealed a significant decrease (P<0.05) for both periods 120 and 144 hrs as compared with control group (C) for the same trait.

Therefore, it was concluded that adding GT infusion to the ram semen extenders can be used to enhance semen characteristics which include motility and viability as well as reduce the abnormality of ram semen stored at 5°