Effect of escherichia coli, salmonella spp., and staphylococcus aureus isolated from polluted drainage water on rats
Joint Authors
Uthman, A. Jamal
Minshawi, Midhat M.
Faraj, Abd al-Raziq H.
Source
Journal of the Arab Society for Medical Research
Issue
Vol. 11, Issue 1 (30 Jun. 2016), pp.1-8, 8 p.
Publisher
Arab Society for Medical Research
Publication Date
2016-06-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Biology
Earth Sciences, Water and Environment
Topics
Abstract EN
Background/aim Water pollution not only damages the environment but also kills wildlife.
The present study aimed to investigate the effect of Escherichia coli, Salmonella typhi, and Staphylococcus aureus, which were isolated from drainage water at El-Rahawey area,Giza, Egypt, on experimental rats.
Materials and methods Water samples were collected from the subsurface layer for bacteriologicalexamination, stored in an ice box, and delivered to the laboratory and analyzed to count the total viable bacterial counts/ml using the most probable number technique.
The isolated bacteria were given to rats for 21 days at a dose equal to 103/ml and the liver and kidney were dissected for histopathological and histochcemical studies.
Results Results showed that the average counts at 22 and 37°C were 105 and 106CFU/ml, respectively, whereas the average counts by using the most probable number technique/100 ml were 105, 104, 103, 103, and 102 for total coliforms, fecal coliforms, E.
coli, Salmonellae group, and total staphylococci, respectively.
Isolation of pathogens from water sources were identified by using membrane filter technique on specific media.
The histopathological examination of the liver of treated rats with E.
coli, S.
typhi, and S.
aureus revealed swollen hepatocytes with decreased sinusoidal spaces and widely distributed necrotic foci.
In the kidney, renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex.
The histochemical study indicated the depletion of staining of protein and polysaccharides.
Conclusion E.
coli, S.
typhi, and S.
aureus that are isolated from polluted drainage water cause histological and histochemical changes in the liver and kidney of rats.
American Psychological Association (APA)
Uthman, A. Jamal& Minshawi, Midhat M.& Faraj, Abd al-Raziq H.. 2016. Effect of escherichia coli, salmonella spp., and staphylococcus aureus isolated from polluted drainage water on rats. Journal of the Arab Society for Medical Research،Vol. 11, no. 1, pp.1-8.
https://search.emarefa.net/detail/BIM-696506
Modern Language Association (MLA)
Minshawi, Midhat M.…[et al.]. Effect of escherichia coli, salmonella spp., and staphylococcus aureus isolated from polluted drainage water on rats. Journal of the Arab Society for Medical Research Vol. 11, no. 1 (Jan. / Jun. 2016), pp.1-8.
https://search.emarefa.net/detail/BIM-696506
American Medical Association (AMA)
Uthman, A. Jamal& Minshawi, Midhat M.& Faraj, Abd al-Raziq H.. Effect of escherichia coli, salmonella spp., and staphylococcus aureus isolated from polluted drainage water on rats. Journal of the Arab Society for Medical Research. 2016. Vol. 11, no. 1, pp.1-8.
https://search.emarefa.net/detail/BIM-696506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-8
Record ID
BIM-696506