Estimating CD56 and perforin in rats primed by breast cancer cell line T47D

Abstract EN

Breast cancer is the most common disease in females and it has become a major health problem affecting women worldwide.

This study was carry out on different groups of female albino rats Rattus rattus, that primed by different forms of antigens prepared from cell line of human breast cancer T47d.

The current study has been designed to determine the immune response of rats for breast cancer cells antigens.

The immune parameters CD56 and perforin have been estimated.

Significant increase (P < 0.05) is found in the level of expression of CD56 on the surfaces of natural killer cells of rats in all treated groups, but higher level in group that injected by mixture of freezing killed antigen with adjuvant that reached to 83.164 ± 7.616 ng/ml is compared with the control groups.

The results have indicated the presence of significant increase in the level of cellular toxin perforin in all treatment groups especially the rats that immunized by freezing killed antigen with adjuvant which reached to 113.45 ± 15.925 ng/ml compared with the healthy rats.