Evaluation of the sperm cervical mucus contact test (SCMC)‎ for assessment of cervical factor infertility

Abstract EN

Background : Cervical mucus is a necessary component of human fertility.

Cervical mucus is considered a main barrier for sperms for passing to the upper female reproductive tract, only less than 1% of the spermatozoa in vagina can penetrate the cervical mucus successfully.

Objective : Use in vitro sperm cervical mucus contact test (SCMC) to evaluate the fertility of couples complaining from unexplained infertility.

Methods : This study was conducted in the Infertility Unit/ Babylon Hospital of Gynecology and Children.

Seminal fluid analysis was performed for all 45 studied specimens.

SCMC test was performed by aspirating a cervical mucus at 12-14 day cycle of females.

One drop of semen mixed with a drop of cervical mucus and covered with cover slide.

The slide is then incubated at 37C° for 30 minutes.The sperm concentration and sperm motility due to grading activity were evaluated and compared with semen parameters.

Results: The results showed a significant decrease (p<0.001) of sperm concentration and forward sperm motility (grade a & b ), while a significant increase (p<0.001) in grade c and d sperm motility compared to their values in seminal fluid analysis.

The correlation (r) study showed significant negative correlation between forward sperm motility a+b with age of females, while a positive correlation between grade c of sperm motility and age of females, and non-significant correlation between all studied sperm parameters with infertility period.

Conclusion : It was concluded that sperm SCMC test is necessary for detecting the causes of infertility for Normospermic men.