Use of PCR to detection Pseudomonas aeruginosa from clinical samples in Hilla Teaching Hospital

Abstract EN

Wounds, burns, urinary tract infection (UTI) and ear infections are often difficult to treat due to various bacterial pathogens.

Pseudomonas aeruginosa is one of the common invaders of this infection .

Precise diagnosis of this etiological agent in this infection is of critical importance particularly in treatment of problematic cases.

The existing diagnostic methods have certain limitations particularly related to specificity.

This study was aimed to reliable diagnosis of P.

aeruginosa from samples enrichment in nutrient broth PCR was used to detect P.

aeruginosa with two primers targeted one gene saved in housekeeping gene (gyr b).

The first primer (gyr b190) amplified 190-bp fragment , whereas other bacteria did not yield any 190-bp fragment.

The specificity of the assay was 100% .

PCR method depending on primer gyr b190 is rapid and more accurate than other diagnostic methods for the identification of P.

aeruginosa strains, and it can be used to detect P.

aeruginosa from clinical samples without using a selective medium or additional biochemical tests.

While second primer gyr b222 amplified gyr b with fragment 222bp only when using a selective medium, but in nutrient broth get false negative.